Genetics


Publications
355

Circular metagenome-assembled genome of Candidatus Patescibacteria recovered from anaerobic digestion sludge

Citation
Sakurai et al. (2024). Microbiology Resource Announcements 13 (4)
Names
Abstract
ABSTRACT A single-contig, circular metagenome-assembled genome (cMAG) of Candidatus ( Ca. ) Patescibacteria was reconstructed from a mesophilic full-scale food waste treatment plant in Japan. The genome is of small size and lacks fundamental biosynthetic pathways. Taxonomic analysis using the Genome Taxonomy Database revealed that this cMAG belonged to the genus JAEZRQ01 ( Ca

Integrated bacterial transcriptome and host metabolome analysis reveals insights into “ Candidatus Liberibacter asiaticus” population dynamics in the fruit pith of three citrus cultivars with different tolerance

Citation
Li et al. (2024). Microbiology Spectrum 12 (4)
Names
Ca. Liberibacter asiaticus
Abstract
ABSTRACT “ Candidatus Liberibacter asiaticus” (CLas), the causal agent of citrus Huanglongbing (HLB), is able to multiply to a high abundance in citrus fruit pith. However, little is known about the biological processes and phytochemical substances that are vital for CLas colonization and growth in fruit pith. In this study, CLas-infected fruit pith of three citrus cultivars (“Shatangju” mandarin, “Guanxi” pomelo, and

Refinement of the “ Candidatus Accumulibacter” genus based on metagenomic analysis of biological nutrient removal (BNR) pilot-scale plants operated with reduced aeration

Citation
Stewart et al. (2024). mSystems 9 (3)
Names
“Accumulibacter”
Abstract
ABSTRACT Members of the “ Candidatus Accumulibacter” genus are widely studied as key polyphosphate-accumulating organisms (PAOs) in biological nutrient removal (BNR) facilities performing enhanced biological phosphorus removal (EBPR). This diverse lineage includes 18 “ Ca . Accumulibacter” species, which have been proposed based on the phylogenetic divergence of the polyphosphate kinase 1 (

Towards the completion of Koch's postulates for the citrus huanglongbing bacterium, Candidatus Liberibacter asiaticus

Citation
Zheng et al. (2024). Horticulture Research 11 (3)
Names
Ca. Liberibacter asiaticus
Abstract
Abstract Candidatus Liberibacter asiaticus (Las) is one of the causal agents of huanglongbing (HLB), the most devastating disease of citrus worldwide. Due to the intracellular lifestyle and significant genome reduction, culturing Las in vitro has proven to be extremely challenging. In this study, we optimized growth conditions and developed a semi-selective medium based on the results of nutritional and antibiotic screening assays. Using these optimized conditions, we were able to

Molecular detection of ‘Candidatus phytoplasma aurantifolia’ associated with virescence and phyllody of Zinnia peruviana: A new record for India

Citation
Mahadevakumar et al. (2024). Journal of Phytopathology 172 (2)
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Abstract
AbstractZinnia peruviana (Asteraceae) is an annual flowering plant cultivated in various gardens of Mysore, the south of Karnataka state, India. During 2021, phytoplasma‐like symptoms such as virescence and phyllody were observed on Zinnia in Mysore local gardens. A total of 10 symptomatic and five asymptomatic samples were collected, and the DNA was amplified by PCR using primers specific to 16S RNA and secA genes. The nucleotide sequence analysis showed that the 16S RNA and SecA gene sequences

Candidatus Methanosphaera massiliense sp. nov., a methanogenic archaeal species found in a human fecal sample and prevalent in pigs and red kangaroos

Citation
Pilliol et al. (2024). Microbiology Spectrum 12 (2)
Names
Ca. Methanosphaera massiliense
Abstract
ABSTRACT Methanosphaera stadtmanae was the sole Methanosphaera representative to be cultured and detected by molecular methods in the human gut microbiota, further associated with digestive and respiratory diseases, leaving unknown the actual diversity of human-associated Methanosphaera species. Here, a novel Methanosphaera species, Candidatus Methanosphaera massilie

ABA-CsABI5-CsCalS11 module upregulates Callose deposition of citrus infected with Candidatus Liberibacter asiaticus

Citation
Yao et al. (2024). Horticulture Research 11 (2)
Names
Ca. Liberibacter asiaticus
Abstract
Abstract Huanglongbing (HLB) primarily caused by Candidatus Liberibacter asiaticus (CLas) has been threatening citrus production globally. Under HLB conditions, an excessive accumulation of the polysaccharide callose in citrus phloem occurs, leading to phloem blockage and starch accumulation in leaves. The callose production is controlled by callose synthases (CalS), which have multiple members within plants. However, the knowledge of callose production in the citrus upon infectio

Molecular characterization of ‘Candidatus Phytoplasma phoenicium’ infecting almond (Prunus dulcis) and evaluation of biochemical defenses produced in the plants

Citation
Akkurak et al. (2024). Journal of Phytopathology 172 (1)
Names
Ca. Phytoplasma phoenicium
Abstract
AbstractIncreasing incidences of phytoplasma infestations in Almond trees warrants the better management approach to prevent yield losses. Disease management rely on identification of the pathogen based on molecular profiling. The present study aimed, to identify the phytoplasma agent in almond trees and to measure the biochemical responses it causes in the host. Direct and Nested PCRs performed using phytoplasma specific primer pairs 16S rRNA, detected the presence of phytoplasma agent in sympt