Genetics


Publications (271)

Mutual Exclusion of <i>Methanobrevibacter</i> Species in the Human Gut Microbiota Facilitates Directed Cultivation of a <i>Candidatus</i> Methanobrevibacter Intestini Representative

Citation
Low et al. (2022). Microbiology Spectrum 10 (4)
Names
Methanobrevibacter
Subjects
Cell Biology Ecology General Immunology and Microbiology Genetics Infectious Diseases Microbiology (medical) Physiology
Abstract
Methanogens are important hydrogen consumers in the gut and are associated with differing host health. Here, we determine the prevalence and abundance of archaeal species in the guts of a multi-ethnic cohort of healthy Singapore residents.

Draft Genome Sequences of Nine “ <i>Candidatus</i> Nanosynbacter sp. HMT-352” Strains Cultured from the Human Oral Cavity

Citation
Liu et al. (2022). Microbiology Resource Announcements 11 (8)
Names
Ca. Saccharibacteria Ca. Nanosynbacter
Subjects
Genetics Immunology and Microbiology (miscellaneous) Molecular Biology
Abstract
Here, we report draft genome sequences for nine strains of “ Candidatus Nanosynbacter sp. HMT-352.” These strains and their sequences were used to interrogate strain-level variations in host range, gene content, and growth dynamics among the phylum “ Candidatus Saccharibacteria.”

Identification of housekeeping genes of Candidatus Branchiomonas cysticola associated with epitheliocystis in Atlantic salmon (Salmo salar L.)

Citation
Mjølnerød et al. (2022). Archives of Microbiology 204 (7)
Names
Ca. Branchiomonas cysticola
Subjects
Biochemistry General Medicine Genetics Microbiology Molecular Biology
Abstract
AbstractCandidatus Branchiomonas cysticola is an intracellular, gram-negative Betaproteobacteria causing epitheliocystis in Atlantic Salmon (Salmo salar L.). The bacterium has not been genetically characterized at the intraspecific level despite its high prevalence among salmon suffering from gill disease in Norwegian aquaculture. DNA from gill samples of Atlantic salmon PCR positive for Cand. B. cysticola and displaying pathological signs of gill disease, was, therefore, extracted and subject to next-generation sequencing (mNGS). Partial sequences of four housekeeping (HK) genes (aceE, lepA, rplB, rpoC) were ultimately identified from the sequenced material. Assays for real-time RT-PCR and fluorescence in-situ hybridization, targeting the newly acquired genes, were simultaneously applied with existing assays targeting the previously characterized 16S rRNA gene. Agreement in both expression and specificity between these putative HK genes and the 16S gene was observed in all instances, indicating that the partial sequences of these HK genes originate from Cand. B. cysticola. The knowledge generated from the present study constitutes a major prerequisite for the future design of novel genotyping schemes for this bacterium.

A closed Candidatus Odinarchaeum chromosome exposes Asgard archaeal viruses

Citation
Tamarit et al. (2022). Nature Microbiology 7 (7)
Names
Ca. Odinarchaeum yellowstonii
Subjects
Applied Microbiology and Biotechnology Cell Biology Genetics Immunology Microbiology Microbiology (medical)
Abstract
AbstractAsgard archaea have recently been identified as the closest archaeal relatives of eukaryotes. Their ecology, and particularly their virome, remain enigmatic. We reassembled and closed the chromosome of Candidatus Odinarchaeum yellowstonii LCB_4, through long-range PCR, revealing CRISPR spacers targeting viral contigs. We found related viruses in the genomes of diverse prokaryotes from geothermal environments, including other Asgard archaea. These viruses open research avenues into the ecology and evolution of Asgard archaea.

Reevaluation of the Phylogenetic Diversity and Global Distribution of the Genus “ <i>Candidatus</i> Accumulibacter”

Citation
Petriglieri et al. (2022). mSystems 7 (3)
Names
Ca. Accumulibacter
Subjects
Biochemistry Computer Science Applications Ecology, Evolution, Behavior and Systematics Genetics Microbiology Modeling and Simulation Molecular Biology Physiology
Abstract
“ Candidatus Accumulibacter” is the most studied PAO, with a primary role in biological nutrient removal. However, the species-level taxonomy of this lineage is convoluted due to the use of different phylogenetic markers or genome sequencing approaches. Here, we redefined the phylogeny of these organisms, proposing a comprehensive approach which could be used to address the classification of other diverse and uncultivated lineages.

Complete Genome Sequence of “ <i>Candidatus</i> Nanosynbacter” Strain HMT-348_TM7c-JB, a Member of <i>Saccharibacteria</i> Clade G1

Citation
Baker (2022). Microbiology Resource Announcements 11 (5)
Names
Ca. Nanosynbacter
Subjects
Genetics Immunology and Microbiology (miscellaneous) Molecular Biology
Abstract
Saccharibacteria are abundant and diverse members of the human oral microbiome; however, they are poorly understood and appear to exhibit an epibiont/parasitic lifestyle dependent on host bacteria. Here, a complete metagenome-assembled genome (MAG) sequence of an organism from Saccharibacteria clade G1 human microbial taxon (HMT) 348 is reported, strain HMT-348_TM7c-JB.