Biochemistry


Publications
98

Refinement of the “ Candidatus Accumulibacter” genus based on metagenomic analysis of biological nutrient removal (BNR) pilot-scale plants operated with reduced aeration

Citation
Stewart et al. (2024). mSystems 9 (3)
Names
“Accumulibacter”
Abstract
ABSTRACT Members of the “ Candidatus Accumulibacter” genus are widely studied as key polyphosphate-accumulating organisms (PAOs) in biological nutrient removal (BNR) facilities performing enhanced biological phosphorus removal (EBPR). This diverse lineage includes 18 “ Ca . Accumulibacter” species, which have been proposed based on the phylogenetic divergence of the polyphosphate kinase 1 (

Towards the completion of Koch's postulates for the citrus huanglongbing bacterium, Candidatus Liberibacter asiaticus

Citation
Zheng et al. (2024). Horticulture Research 11 (3)
Names
Ca. Liberibacter asiaticus
Abstract
Abstract Candidatus Liberibacter asiaticus (Las) is one of the causal agents of huanglongbing (HLB), the most devastating disease of citrus worldwide. Due to the intracellular lifestyle and significant genome reduction, culturing Las in vitro has proven to be extremely challenging. In this study, we optimized growth conditions and developed a semi-selective medium based on the results of nutritional and antibiotic screening assays. Using these optimized conditions, we were able to

ABA-CsABI5-CsCalS11 module upregulates Callose deposition of citrus infected with Candidatus Liberibacter asiaticus

Citation
Yao et al. (2024). Horticulture Research 11 (2)
Names
Ca. Liberibacter asiaticus
Abstract
Abstract Huanglongbing (HLB) primarily caused by Candidatus Liberibacter asiaticus (CLas) has been threatening citrus production globally. Under HLB conditions, an excessive accumulation of the polysaccharide callose in citrus phloem occurs, leading to phloem blockage and starch accumulation in leaves. The callose production is controlled by callose synthases (CalS), which have multiple members within plants. However, the knowledge of callose production in the citrus upon infectio

A comprehensive overview of the Chloroflexota community in wastewater treatment plants worldwide

Citation
Petriglieri et al. (2023). mSystems 8 (6)
Names
“Epilinea brevis” “Epilinea” “Leptofilum” “Epilineaceae” “Epilineales” “Avedoeria danica” “Avedoeria” “Brachythrix odensensis” “Brachythrix” “Defluviilinea gracilis” “Defluviilinea” “Defluviilinea proxima” “Villigracilis vicinus” “Villigracilis adiacens” “Villigracilis propinquus” “Villigracilis” “Villigracilis affinis” “Villigracilis proximus” “Villigracilis saccharophilus” “Villigracilaceae” “Hadersleviella danica” “Hadersleviella” “Trichofilum aggregatum” “Trichofilum” “Promineofilum glycogenicum” “Leptofilum gracile” “Leptofilum proximum” “Leptovillus gracilis” “Leptovillus affinis” “Leptovillus” “Flexicrinis affinis” “Flexicrinis proximus” “Flexicrinis” “Flexifilum breve” “Flexifilum affine” “Flexifilum” “Flexifilaceae” “Amarolinea dominans” “Fredericiella danica” “Fredericiella” “Caldilinea saccharophila” “Ribeiella danica” “Ribeiella” “Kouleothrix ribensis” “Amarobacter glycogenicus” “Amarobacter” “Amarobacillus elongatus” “Amarobacillus”
Abstract
ABSTRACT Filamentous Chloroflexota are abundant in activated sludge wastewater treatment plants (WWTPs) worldwide and are occasionally associated with poor solid-liquid separation or foaming, but most of the abundant lineages remain undescribed. Here, we present a comprehensive overview of Chloroflexota abundant in WWTPs worldwide, using high-quality metagenome-assembled genomes (MAGs) and 16S rRNA amplicon data from 740 Danish and global WWTPs. Many novel taxa were descri

Oxygen Uptake Rate as an Indicator of the Substrates Utilized by Candidatus Accumulibacter

Citation
Dorofeev et al. (2023). Water 15 (20)
Names
“Accumulibacter”
Abstract
Candidatus Accumulibacter belongs to phosphate-accumulating organisms (PAOs) which exhibit a cyclic metabolism and are capable of intracellular polyphosphate accumulation and their hydrolysis under feast-famine anaerobic-aerobic cycling. In consortia of activated sludge microorganisms, these bacteria are responsible for enhanced biological phosphorus removal (EBPR). The spectrum of the substrates used by Ca. Accumulibacter remains insufficiently studied. It was investigated by measuring the oxyg

An endolysin gene from Candidatus Liberibacter asiaticus confers dual resistance to huanglongbing and citrus canker

Citation
Xu et al. (2023). Horticulture Research 10 (9)
Names
Ca. Liberibacter asiaticus
Abstract
Abstract The most damaging citrus diseases are Huanglongbing (HLB) and citrus canker, which are caused by Candidatus Liberibacter asiaticus (CaLas) and Xanthomonas citri pv. citri (Xcc), respectively. Endolysins from bacteriophages are a possible option for disease resistance in plant breeding. Here, we report improvement of citrus resistance to HLB and citrus canker using the LasLYS1 and LasLYS2 endolysins from CaLas. LasLYS2 demonstrated bactericidal efficacy against several Rhi