Molecular Biology


Publications
311

Refinement of the “ Candidatus Accumulibacter” genus based on metagenomic analysis of biological nutrient removal (BNR) pilot-scale plants operated with reduced aeration

Citation
Stewart et al. (2024). mSystems
Names
“Accumulibacter”
Abstract
ABSTRACT Members of the “ Candidatus Accumulibacter” genus are widely studied as key polyphosphate-accumulating organisms (PAOs) in biological nutrient removal (BNR) facilities performing enhanced biological phosphorus removal (EBPR). This diverse lineage includes 18 “ Ca . Accumulibacter” species, which have been proposed based on the phylogenetic divergence of the polyphosphate kinase 1 (

A comprehensive overview of the Chloroflexota community in wastewater treatment plants worldwide

Citation
Petriglieri et al. (2023). mSystems 8 (6)
Names
“Flexifilaceae” “Flexifilum” “Flexifilum affine” “Flexifilum breve” “Flexicrinis” “Flexicrinis proximus” “Flexicrinis affinis” “Leptovillus” “Leptovillus affinis” “Leptovillus gracilis” “Leptofilum” “Leptofilum proximum” “Leptofilum gracile” “Promineofilum glycogenicum” “Trichofilum” “Trichofilum aggregatum” “Hadersleviella” “Hadersleviella danica” “Villigracilaceae” “Villigracilis saccharophilus” “Villigracilis proximus” “Villigracilis affinis” “Villigracilis” “Villigracilis propinquus” “Villigracilis adiacens” “Villigracilis vicinus” “Defluviilinea proxima” “Defluviilinea” “Defluviilinea gracilis” “Brachythrix” “Brachythrix odensensis” “Avedoeria” “Avedoeria danica” “Epilineales” “Epilineaceae” “Epilinea” “Epilinea brevis”
Abstract
ABSTRACT Filamentous Chloroflexota are abundant in activated sludge wastewater treatment plants (WWTPs) worldwide and are occasionally associated with poor solid-liquid separation or foaming, but most of the abundant lineages remain undescribed. Here, we present a comprehensive overview of Chloroflexota abundant in WWTPs worldwide, using high-quality metagenome-assembled genomes (MAGs) and 16S rRNA amplicon data from 740 Danish and global WWTPs. Many novel taxa were descri

Complete genome sequence of “ Candidatus Phytoplasma cynodontis” GY2015, a plant pathogen associated with Bermuda grass white leaf disease in Taiwan

Citation
Cho et al. (2023). Microbiology Resource Announcements 12 (10)
Names
Ca. Phytoplasma cynodontis
Abstract
ABSTRACT The complete genome sequence of “ Candidatus Phytoplasma cynodontis” strain GY2015, which consists of one 498,922-bp circular chromosome, is presented in this work. This uncultivated plant-pathogenic bacterium is associated with Bermuda grass white leaf disease in Taoyuan, Taiwan.

Complete genome sequence of Candidatus Mycobacterium wuenschmannii , a nontuberculous mycobacterium isolated from a captive population of Amazon milk frogs

Citation
Zeineldin et al. (2023). Microbiology Resource Announcements 12 (10)
Names
Ca. Mycobacterium wuenschmannii
Abstract
ABSTRACT A slow growing species of nontuberculous mycobacteria (NTM) was isolated from the liver of an Amazon milk frog. The complete genome of this isolate comprises 5,102,433 bp, exhibiting 66.86% GC content, 4,940 protein-coding sequences, 52 predicted RNA genes, and 39 repeat regions.

Complete genomes of mutualistic bacterial co-symbionts “ Candidatus Sulcia muelleri” and “ Candidatus Nasuia deltocephalinicola” of the rice green leafhopper Nephotettix cincticeps

Citation
Moriyama et al. (2023). Microbiology Resource Announcements 12 (9)
Names
Ca. Sulcia muelleri Ca. Nasuia deltocephalinicola
Abstract
ABSTRACT The genomes of obligate bacterial co-symbionts of the green rice leafhopper Nephotettix cincticeps , which is notorious as an agricultural pest, were determined. The streamlined genomes of “ Candidatus Sulcia muelleri” and “ Candidatus Nasuia deltocephalinicola” exhibited complementary metabolic pathways for synthesizing essential nutrients that contribute to host adaptation.

Complete genome sequence of “ Candidatus Phytoplasma pruni” PR2021, an uncultivated bacterium associated with poinsettia ( Euphorbia pulcherrima )

Citation
Pei et al. (2023). Microbiology Resource Announcements 12 (9)
Names
Ca. Phytoplasma pruni
Abstract
ABSTRACT The complete genome sequence of “ Candidatus Phytoplasma pruni” strain PR2021, which consists of one 705,138 bp circular chromosome and one 4,757 bp circular plasmid, is presented in this work. This bacterium is associated with poinsettia ( Euphorbia pulcherrima ) cultivar “Princettia Pink.”

Metagenomics Revealed a New Genus ‘Candidatus Thiocaldithrix dubininis’ gen. nov., sp. nov. and a New Species ‘Candidatus Thiothrix putei’ sp. nov. in the Family Thiotrichaceae, Some Members of Which Have Traits of Both Na+- and H+-Motive Energetics

Citation
Ravin et al. (2023). International Journal of Molecular Sciences 24 (18)
Names
Ca. Thiothrix putei Ca. Thiocaldithrix dubininis
Abstract
Two metagenome-assembled genomes (MAGs), GKL-01 and GKL-02, related to the family Thiotrichaceae have been assembled from the metagenome of bacterial mat obtained from a sulfide-rich thermal spring in the North Caucasus. Based on average amino acid identity (AAI) values and genome-based phylogeny, MAG GKL-01 represented a new genus within the Thiotrichaceae family. The GC content of the GKL-01 DNA (44%) differed significantly from that of other known members of the genus Thiothrix (50.1–55.6%).