Molecular Biology


Publications (297)

Complete genome sequence of Candidatus Mycobacterium wuenschmannii , a nontuberculous mycobacterium isolated from a captive population of Amazon milk frogs

Citation
Zeineldin et al. (2023). Microbiology Resource Announcements
Names (1)
Ca. Mycobacterium wuenschmannii
Subjects
Genetics Immunology and Microbiology (miscellaneous) Molecular Biology
Abstract
ABSTRACT A slow growing species of nontuberculous mycobacteria (NTM) was isolated from the liver of an Amazon milk frog. The complete genome of this isolate comprises 5,102,433 bp, exhibiting 66.86% GC content, 4,940 protein-coding sequences, 52 predicted RNA genes, and 39 repeat regions.

Complete genomes of mutualistic bacterial co-symbionts “ Candidatus Sulcia muelleri” and “ Candidatus Nasuia deltocephalinicola” of the rice green leafhopper Nephotettix cincticeps

Citation
Moriyama et al. (2023). Microbiology Resource Announcements 12 (9)
Names (2)
Ca. Sulcia muelleri Ca. Nasuia deltocephalinicola
Subjects
Genetics Immunology and Microbiology (miscellaneous) Molecular Biology
Abstract
ABSTRACT The genomes of obligate bacterial co-symbionts of the green rice leafhopper Nephotettix cincticeps , which is notorious as an agricultural pest, were determined. The streamlined genomes of “ Candidatus Sulcia muelleri” and “ Candidatus Nasuia deltocephalinicola” exhibited complementary metabolic pathways for synthesizing essential nutrients that contribute to host adaptation.

Complete genome sequence of “ Candidatus Phytoplasma pruni” PR2021, an uncultivated bacterium associated with poinsettia ( Euphorbia pulcherrima )

Citation
Pei et al. (2023). Microbiology Resource Announcements 12 (9)
Names (1)
Ca. Phytoplasma pruni
Subjects
Genetics Immunology and Microbiology (miscellaneous) Molecular Biology
Abstract
ABSTRACT The complete genome sequence of “ Candidatus Phytoplasma pruni” strain PR2021, which consists of one 705,138 bp circular chromosome and one 4,757 bp circular plasmid, is presented in this work. This bacterium is associated with poinsettia ( Euphorbia pulcherrima ) cultivar “Princettia Pink.”

Metagenomics Revealed a New Genus ‘Candidatus Thiocaldithrix dubininis’ gen. nov., sp. nov. and a New Species ‘Candidatus Thiothrix putei’ sp. nov. in the Family Thiotrichaceae, Some Members of Which Have Traits of Both Na+- and H+-Motive Energetics

Citation
Ravin et al. (2023). International Journal of Molecular Sciences 24 (18)
Names (2)
Ca. Thiothrix putei Ca. Thiocaldithrix dubininis
Subjects
Catalysis Computer Science Applications General Medicine Inorganic Chemistry Molecular Biology Organic Chemistry Physical and Theoretical Chemistry Spectroscopy
Abstract
Two metagenome-assembled genomes (MAGs), GKL-01 and GKL-02, related to the family Thiotrichaceae have been assembled from the metagenome of bacterial mat obtained from a sulfide-rich thermal spring in the North Caucasus. Based on average amino acid identity (AAI) values and genome-based phylogeny, MAG GKL-01 represented a new genus within the Thiotrichaceae family. The GC content of the GKL-01 DNA (44%) differed significantly from that of other known members of the genus Thiothrix (50.1–55.6%). We proposed to assign GKL-01 to a new species and genus ‘Candidatus Thiocaldithrix dubininis’ gen. nov., sp. nov. GKL-01. The phylogenetic analysis and estimated distances between MAG GKL-02 and the genomes of the previously described species of the genus Thiothrix allowed assigning GKL-02 to a new species with the proposed name ‘Candidatus Thiothrix putei’ sp. nov. GKL-02 within the genus Thiothrix. Genome data first revealed the presence of both Na+-ATPases and H+-ATPases in several Thiothrix species. According to genomic analysis, bacteria GKL-01 and GKL-02 are metabolically versatile facultative aerobes capable of growing either chemolithoautotrophically or chemolithoheterotrophically in the presence of hydrogen sulfide and/or thiosulfate or chemoorganoheterotrophically.

Complete genome sequence of “ Candidatus Phytoplasma cynodontis” GY2015, a plant pathogen associated with Bermuda grass white leaf disease in Taiwan

Citation
Cho et al. (2023). Microbiology Resource Announcements
Names (1)
Ca. Phytoplasma cynodontis
Subjects
Genetics Immunology and Microbiology (miscellaneous) Molecular Biology
Abstract
ABSTRACT The complete genome sequence of “ Candidatus Phytoplasma cynodontis” strain GY2015, which consists of one 498,922-bp circular chromosome, is presented in this work. This uncultivated plant-pathogenic bacterium is associated with Bermuda grass white leaf disease in Taoyuan, Taiwan.

Mycoplasma hominis and Candidatus Mycoplasma girerdii in Trichomonas vaginalis: Peaceful Cohabitants or Contentious Roommates?

Citation
Margarita et al. (2023). Pathogens 12 (9)
Names (1)
Ca. Mycoplasma girerdii
Subjects
General Immunology and Microbiology Immunology and Allergy Infectious Diseases Microbiology (medical) Molecular Biology
Abstract
Trichomonas vaginalis is a pathogenic protozoan diffused worldwide capable of infecting the urogenital tract in humans, causing trichomoniasis. One of its most intriguing aspects is the ability to establish a close relationship with endosymbiotic microorganisms: the unique association of T. vaginalis with the bacterium Mycoplasma hominis represents, to date, the only example of an endosymbiosis involving two true human pathogens. Since its discovery, several aspects of the symbiosis between T. vaginalis and M. hominis have been characterized, demonstrating that the presence of the intracellular guest strongly influences the pathogenic characteristics of the protozoon, making it more aggressive towards host cells and capable of stimulating a stronger proinflammatory response. The recent description of a further symbiont of the protozoon, the newly discovered non-cultivable mycoplasma Candidatus Mycoplasma girerdii, makes the picture even more complex. This review provides an overview of the main aspects of this complex microbial consortium, with particular emphasis on its effect on protozoan pathobiology and on the interplays among the symbionts.

Association of SFG Rickettsia massiliae and Candidatus Rickettsia shennongii with Different Hard Ticks Infesting Livestock Hosts

Citation
Shehla et al. (2023). Pathogens 12 (9)
Names (1)
Ca. Rickettsia shennongii
Subjects
General Immunology and Microbiology Immunology and Allergy Infectious Diseases Microbiology (medical) Molecular Biology
Abstract
Ixodid ticks are responsible for the transmission of various intracellular bacteria, such as the Rickettsia species. Little Information is available about the genetic characterization and epidemiology of Rickettsia spp. The current study was designed to assess the tick species infesting various livestock hosts and the associated Rickettsia spp. in Pakistan. Ticks were collected from different livestock hosts (equids, cattle, buffaloes, sheep, goats, and camels); morphologically identified; and screened for the genetic characterization of Rickettsia spp. by the amplification of partial fragments of the gltA, ompA and ompB genes. Altogether, 707 ticks were collected from 373 infested hosts out of 575 observed hosts. The infested hosts comprised 105 cattle, 71 buffaloes, 70 sheep, 60 goats, 34 camels, and 33 equids. The overall occurrence of Rickettsia spp. was 7.6% (25/330) in the tested ticks. Rickettsia DNA was detected in Rhipicephalus haemaphysaloides (9/50, 18.0%), followed by Rhipicephalus turanicus (13/99, 13.1%), Haemaphysalis cornupunctata (1/18, 5.5%), and Rhipicephalus microplus (2/49, 4.1%); however, no rickettsial DNA was detected in Hyalomma anatolicum (71), Hyalomma dromedarii (35), and Haemaphysalis sulcata (8). Two Rickettsia agents were identified based on partial gltA, ompA, and ompB DNA sequences. The Rickettsia species detected in Rh. haemaphysaloides, Rh. turanicus, and Rh. microplus showed 99–100% identity with Rickettsia sp. and Candidatus Rickettsia shennongii, and in the phylogenetic trees clustered with the corresponding Rickettsia spp. The Rickettsia species detected in Rh. haemaphysaloides, Rh. turanicus, Rh. microplus, and Ha. cornupunctata showed 100% identity with R. massiliae, and in the phylogenetic trees it was clustered with the same species. Candidatus R. shennongii was characterized for the first time in Rh. haemaphysaloides, Rh. turanicus, and Rh. microplus. The presence of SFG Rickettsia spp., including the human pathogen R. massiliae, indicates a zoonotic risk in the study region, thus stressing the need for regular surveillance.

Proposal of names for 329 higher rank taxa defined in the Genome Taxonomy Database under two prokaryotic codes

Citation
Chuvochina et al. (2023). FEMS Microbiology Letters
Names (42)
“Kapaibacteriia” “Cloacimonadaceae” “Cloacimonadales” “Cloacimonadia” “Methylomirabilota” “Desulforudaceae” “Thermobaculales” “Thermobaculaceae” “Tenderiales” “Tenderiaceae” “Saccharimonadales” “Saccharimonadaceae” “Puniceispirillales” “Puniceispirillaceae” “Pseudothioglobaceae” “Promineifilales” “Promineifilaceae” “Obscuribacteraceae” “Nucleicultricaceae” “Muiribacteriia” “Muiribacteriales” “Muiribacteriaceae” “Methylomirabilia” “Methylomirabilales” “Methylomirabilaceae” “Magnetobacteriaceae” “Kapaibacteriales” “Kapaibacteriaceae” “Johnevansiales” “Johnevansiaceae” “Hepatoplasmataceae” “Hepatobacteraceae” “Bipolaricaulia” “Bipolaricaulaceae” “Bipolaricaulales” “Azobacteroidaceae” “Hydrothermaceae” “Hydrothermales” “Hydrothermia” “Binatia” “Binatales” “Binataceae”
Subjects
Genetics Microbiology Molecular Biology
Abstract
Abstract The Genome Taxonomy Database (GTDB) is a taxonomic framework that defines prokaryotic taxa as monophyletic groups in concatenated protein reference trees according to systematic criteria. This has resulted in a substantial number of changes to existing classifications (https://gtdb.ecogenomic.org). In the case of union of taxa, GTDB names were applied based on the priority of publication. The division of taxa or change in rank led to the formation of new Latin names above the rank of genus that were only made publicly available via the GTDB website without associated published taxonomic descriptions. This has sometimes led to confusion in the literature and databases. A number of the provisional GTDB names were later published in other studies, while many still lack authorships. To reduce further confusion, here we propose names and descriptions for 329 GTDB-defined prokaryotic taxa, 223 of which are suitable for validation under the International Code of Nomenclature of Prokaryotes (ICNP) and 49 under the Code of Nomenclature of Prokaryotes Described from Sequence Data (SeqCode). For the latter we designated 23 genomes as type material. An additional 57 taxa that do not currently satisfy the validation criteria of either code are proposed as Candidatus.

Complete Genome Sequence of “ Candidatus Phytoplasma aurantifolia” TB2022, a Plant Pathogen Associated with Sweet Potato Little Leaf Disease in China

Citation
Li et al. (2023). Microbiology Resource Announcements 12 (7)
Names (1)
Ca. Phytoplasma aurantifolia
Subjects
Genetics Immunology and Microbiology (miscellaneous) Molecular Biology
Abstract
The complete genome sequence of “ Candidatus Phytoplasma aurantifolia” TB2022, which consists of one 670,073-bp circular chromosome, is presented in this work. This bacterium is associated with sweet potato little leaf disease in Fujian Province, China.