Molecular Biology


Publications
314

Identification of housekeeping genes of Candidatus Branchiomonas cysticola associated with epitheliocystis in Atlantic salmon (Salmo salar L.)

Citation
Mjølnerød et al. (2022). Archives of Microbiology 204 (7)
Names
Ca. Branchiomonas cystocola
Abstract
AbstractCandidatus Branchiomonas cysticola is an intracellular, gram-negative Betaproteobacteria causing epitheliocystis in Atlantic Salmon (Salmo salar L.). The bacterium has not been genetically characterized at the intraspecific level despite its high prevalence among salmon suffering from gill disease in Norwegian aquaculture. DNA from gill samples of Atlantic salmon PCR positive for Cand. B. cysticola and displaying pathological signs of gill disease, was, therefore, extracted and subject t

Reevaluation of the Phylogenetic Diversity and Global Distribution of the Genus “CandidatusAccumulibacter”

Citation
Petriglieri et al. (2022). mSystems 7 (3)
Names
“Accumulibacter” “Accumulibacter adiacens” “Accumulibacter meliphilus” “Accumulibacter propinquus” “Accumulibacter contiguus” “Accumulibacter vicinus” “Accumulibacter cognatus” “Accumulibacter affinis” “Accumulibacter proximus” “Accumulibacter necessarius” “Accumulibacter iunctus” “Accumulibacter similis” “Accumulibacter conexus” “Propionivibrio dominans” “Accumulibacter adjunctus” “Proximibacter danicus” “Proximibacter”
Abstract
“CandidatusAccumulibacter” is the most studied PAO, with a primary role in biological nutrient removal. However, the species-level taxonomy of this lineage is convoluted due to the use of different phylogenetic markers or genome sequencing approaches. Here, we redefined the phylogeny of these organisms, proposing a comprehensive approach which could be used to address the classification of other diverse and uncultivated lineages.

The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection

Citation
Cheng et al. (2022). International Journal of Molecular Sciences 23 (13)
Names
Liberibacter Ca. Liberibacter asiaticus
Abstract
The citrus industry has been threatened by Huanglongbing (HLB) for over a century. Here, an HLB-induced Arabidopsis RPM1-interacting protein 4 (RIN4) homologous gene was cloned from Citrus clementina, and its characteristics and function were analyzed to determine its role during citrus–Candidatus Liberibacter asiaticus (CLas) interactions. Quantitative real-time PCR showed that RIN4 was expressed in roots, stems, leaves and flowers, with the greatest expression level in leaves. Its expression w

Complete Genome Sequence of “ Candidatus Nanosynbacter” Strain HMT-348_TM7c-JB, a Member of Saccharibacteria Clade G1

Citation
Baker (2022). Microbiology Resource Announcements 11 (5)
Names
Ca. Nanosynbacter
Abstract
Saccharibacteria are abundant and diverse members of the human oral microbiome; however, they are poorly understood and appear to exhibit an epibiont/parasitic lifestyle dependent on host bacteria. Here, a complete metagenome-assembled genome (MAG) sequence of an organism from Saccharibacteria clade G1 human microbial taxon (HMT) 348 is reported, strain HMT-348_TM7c-JB.

Amycolatopsis camponoti sp. nov., new tetracenomycin-producing actinomycete isolated from carpenter ant Camponotus vagus

Citation
Zakalyukina et al. (2022). Antonie van Leeuwenhoek 115 (4)
Names
Amycolatopsis camponoti
Abstract
AbstractAn actinobacterial strain A23T, isolated from adult ant Camponotus vagus collected in Ryazan region (Russia) and established as tetracenomycin X producer, was subjected to a polyphasic taxonomic study. Morphological characteristics of this strain included well-branched substrate mycelium and aerial hyphae fragmented into rod-shaped elements. Phylogenetic analyses based on 16S rRNA gene and genome sequences showed that strain A23T was most closely related to Amycolatopsis pretoriensis DSM

The Longitudinal Dividing Bacterium Candidatus Thiosymbion Oneisti Has a Natural Temperature-Sensitive FtsZ Protein with Low GTPase Activity

Citation
Wang et al. (2022). International Journal of Molecular Sciences 23 (6)
Names
Ca. Thiosymbion Ca. Thiosymbion oneisti
Abstract
FtsZ, the bacterial tubulin-homolog, plays a central role in cell division and polymerizes into a ring-like structure at midcell to coordinate other cell division proteins. The rod-shaped gamma-proteobacterium Candidatus Thiosymbion oneisti has a medial discontinuous ellipsoidal “Z-ring.” Ca. T. oneisti FtsZ shows temperature-sensitive characteristics when it is expressed in Escherichia coli, where it localizes at midcell. The overexpression of Ca. T. oneisti FtsZ interferes with cell division a

Genome Sequence of a New “ Candidatus ” Phylum “Dependentiae” Isolate from Chiba, Japan

Citation
Takemura (2022). Microbiology Resource Announcements 11 (2)
Names
“Babelota”
Abstract
Little is known about the bacterial phylum “ Candidatus Dependentiae,” because only three isolates have been reported. Here, I report the isolation and genome sequencing of a new member of this phylum, strain Noda2021. This is the fourth strain isolated from the phylum “ Candidatus Dependentiae.”