Biotechnology


Publications (161)

Effects of reducing, stabilizing, and antibiotic agents on “Candidatus Kuenenia stuttgartiensis”

Citation
Ude et al. (2023). Applied Microbiology and Biotechnology 107 (5-6)
Names
Ca. Kuenenia stuttgartiensis
Subjects
Applied Microbiology and Biotechnology Biotechnology General Medicine
Abstract
Abstract Anaerobic ammon ium oxidizing (anammox) bacteria oxidize ammonium and reduce nitrite, producing N2, and could play a major role in energy-optimized wastewater treatment. However, sensitivity to various environmental conditions and slow growth currently hinder their wide application. Here, we attempted to determine online the effect of environmental stresses on anammox bacteria by using an overnight batch activity test with whole cells, in which anammox activity was calculated by quantifying N2 production via headspace-pressure monitoring. A planktonic mixed culture dominated by “Candidatus Kuenenia stuttgartiensis” strain CSTR1 was cultivated in a 30-L semi-continuous stirring tank reactor. In overnight resting-cell anammox activity tests, oxygen caused strong inhibition of anammox activity, which was reversed by sodium sulfite (30 µM). The tested antibiotics sulfamethoxazole, kanamycin, and ciprofloxacin elicited their effect on a dose-dependent manner; however, strain CSTR1 was highly resistant to sulfamethoxazole. Anammox activity was improved by activated carbon and Fe2O3. Protein expression analysis from resting cells after anammox activity stimulation revealed that NapC/NirT family cytochrome c (KsCSTR_12840), hydrazine synthase, hydrazine dehydrogenase, hydroxylamine oxidase, and nitrate:nitrite oxidoreductase were upregulated, while a putative hydroxylamine oxidoreductase HAO (KsCSTR_49490) was downregulated. These findings contribute to the growing knowledge on anammox bacteria physiology, eventually leading to the control of anammox bacteria growth and activity in real-world application. Key Points • Sulfite additions can reverse oxygen inhibition of the anammox process • Anammox activity was improved by activated carbon and ferric oxide • Sulfamethoxazole marginally affected anammox activity Graphical abstract

Occurrence, Diversity, and Genomes of “ <i>Candidatus</i> Patescibacteria” along the Early Diagenesis of Marine Sediments

Citation
Zhao et al. (2022). Applied and Environmental Microbiology 88 (24)
Names
Ca. Patescibacteria
Subjects
Applied Microbiology and Biotechnology Biotechnology Ecology Food Science
Abstract
Ultrasmall-celled “ Ca. Patescibacteria” have been estimated to account for one-quarter of the total microbial diversity on Earth, the parasitic lifestyle of which may exert a profound control on the overall microbial population size of the local ecosystems. However, their diversity and metabolic functions in marine sediments, one of the largest yet understudied ecosystems on Earth, remain virtually uncharacterized.

Dehalogenation of Chlorinated Ethenes to Ethene by a Novel Isolate, “ <i>Candidatus</i> Dehalogenimonas etheniformans”

Citation
Chen et al. (2022). Applied and Environmental Microbiology 88 (12)
Names
Ca. Dehalogenimonas etheniformans
Subjects
Applied Microbiology and Biotechnology Biotechnology Ecology Food Science
Abstract
Chlorinated ethenes are risk drivers at many contaminated sites, and current bioremediation efforts focus on organohalide-respiring Dehalococcoides mccartyi strains to achieve detoxification. We isolated and characterized the first non- Dehalococcoides bacterium, “ Candidatus Dehalogenimonas etheniformans” strain GP, capable of metabolic reductive dechlorination of TCE, all DCE isomers, and VC to environmentally benign ethene.

<i>Methanosaeta</i> and “ <i>Candidatus</i> Velamenicoccus archaeovorus”

Citation
Kizina et al. (2022). Applied and Environmental Microbiology 88 (7)
Names
“Velamenicoccus” “Velamenicoccus archaeovorus”
Subjects
Applied Microbiology and Biotechnology Biotechnology Ecology Food Science
Abstract
Epibiotic bacteria are known to live on and off bacterial cells. Here, we describe the ultramicrobacterial anaerobic epibiont OP3 LiM living on Archaea and Bacteria .

The “Other” <i>Rickettsiales</i> : an Overview of the Family “ <i>Candidatus</i> Midichloriaceae”

Citation
Giannotti et al. (2022). Applied and Environmental Microbiology 88 (6)
Names
Ca. Midichloriaceae
Subjects
Applied Microbiology and Biotechnology Biotechnology Ecology Food Science
Abstract
Among endosymbiotic bacterial lineages, few are as intensely studied as Rickettsiales , which include the causative agents of spotted fever, typhus, and anaplasmosis. However, an important subgroup called “ Candidatus Midichloriaceae” receives little attention despite accounting for a third of the diversity of Rickettsiales and harboring a wide range of bacteria with unique features, like the ability to infect mitochondria.