Golyshin, Peter N.


Publications
3

Symbiosis between nanohaloarchaeon and haloarchaeon is based on utilization of different polysaccharides

Citation
La Cono et al. (2020). Proceedings of the National Academy of Sciences 117 (33)
Names
“Nanohalobiia” Ca. Nanohalobium constans “Nanohalobiales” “Nanohalobiaceae”
Abstract
Significance We report on cultivation and characterization of an association between Candidatus Nanohalobium constans and its host, the chitinotrophic haloarchaeon Halomicrobium LC1Hm, obtained from a crystallizer pond of marine solar salterns. High-quality nanohaloarchael genome sequence in conjunction with electron- and fluorescence microscopy, growth analysis, and proteomic and metabolomic data revealed mutually beneficial

A Simple and Rapid System for Proteomic Analysis of the Archaeon Candidatus Vulcanisaeta moutnovskia

Citation
Chernyh et al. (2020).
Names
Ca. Vulcanisaeta moutnovskia
Abstract
Abstract This protocol describes a rapid protein extraction method for the archaeon Candidatus Vulcanisaeta moutnovskia, which can be also implemented for other archaea. The utilization of two different methods for protein extraction constitute the main step of the protocol. Method I involves the extraction with a multi-chaotropic lysis buffer containing a non-denaturing zwitterionic detergent, most efficient for extracting cytosolic proteins. Method II involves a denaturing anionic dete