Kogej, Zala


Development and Validation of a New TaqMan Real-Time PCR for Detection of ‘Candidatus Phytoplasma pruni’

Kogej et al. (2020). Pathogens 9 (8)
Ca. Phytoplasma pruni
Phytoplasmas of the 16SrIII group are wide spread, and have a broad plant host range. Among these, ‘Candidatus phytoplasma pruni’ (‘Ca. P. pruni’; phytoplasmas of 16SrIII subgroup A) can cause serious diseases in Prunus species and ‘Ca. P. pruni’-related strains can infect other plant species, including grapevines. In this study, a new real-time PCR detection system was developed for ‘Ca. P. pruni’ using TaqMan chemistry. This test was designed to detect ‘Ca. P. pruni’, by amplifying the species