Bove, J. M.

Publications (3)

Diversity of “ Candidatus Liberibacter asiaticus,” Based on the omp Gene Sequence

Bastianel et al. (2005). Applied and Environmental Microbiology 71 (11)
Ca. Liberibacter asiaticus
Applied Microbiology and Biotechnology Biotechnology Ecology Food Science
ABSTRACT Huanglongbing (yellow dragon disease) is a destructive disease of citrus. The etiological agent is a noncultured, phloem-restricted alpha-proteobacterium, “ Candidatus Liberibacter africanus” in Africa and “ Candidatus Liberibacter asiaticus” in Asia. In this study, we used an omp -based PCR-restriction fragment length polymorphism (RFLP) approach to analyze the genetic variability of “ Ca . Liberibacter asiaticus” isolates. By using five different enzymes, each the 10 isolates tested could be associated with a specific combination of restriction profiles. The results indicate that the species “ Ca . Liberibacter asiaticus,” even within a given region, may comprise several different variants. Thus, omp -based PCR-RFLP analysis is a simple method for detecting and differentiating “ Ca . Liberibacter asiaticus” isolates.