Johnson, Evan G.


Publications
3

Rapid Movement of ‘Candidatus Liberibacter asiaticus’ on ‘Hamlin’ Sweet Orange and ‘Swingle’ Citrumelo Trunks

Citation
Pulici et al. (2023). PhytoFrontiers™ 3 (2)
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing (HLB), caused by phloem-limited ‘ Candidatus Liberibacter asiaticus’ (CLas), is the primary limiting factor of production in most citrus regions of the world. After infection, CLas is transported systemically throughout the phloem tissues following the source-sink movement. Split-root rhizoboxes and one-sided graft inoculation above the split trunk was used to understand if the vertical distance of the inoculum source and different anatomical structures (grafted or seedling trees)

Assessment of the Effect of Thermotherapy on ‘Candidatus Liberibacter asiaticus’ Viability in Woody Tissue of Citrus via Graft-Based Assays and RNA Assays

Citation
Thapa et al. (2021). Phytopathology® 111 (5)
Names
Ca. Liberibacter asiaticus
Abstract
In 2019, citrus production in Florida declined by more than 70%, mostly because of Huanglongbing (HLB), which is caused by the bacterium ‘Candidatus Liberibacter asiaticus’ (CLas). Thermotherapy for HLB-affected trees was proposed as a short-term management solution to maintain field productivity. It was hypothesized that thermotherapy could eliminate HLB from affected branches; therefore, the study objectives were to show which time–temperature combinations eliminated CLas from woody tissues.

Viability of ‘Candidatus Liberibacter asiaticus’ Prolonged by Addition of Citrus Juice to Culture Medium

Citation
Parker et al. (2014). Phytopathology® 104 (1)
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing, or citrus greening disease, is associated with infection by the phloem-limited bacterium ‘Candidatus Liberibacter asiaticus’. Infection with ‘Ca. L. asiaticus’ is incurable; therefore, knowledge regarding ‘Ca. L. asiaticus’ biology and pathogenesis is essential to develop a treatment. However, ‘Ca. L. asiaticus’ cannot currently be successfully cultured, limiting its study. To gain insight into the conditions conducive for growth of ‘Ca. L. asiaticus’ in vitro, ‘Ca. L. asiaticus’