Biotechnology


Publications
188

Intact Membrane Lipids of “ Candidatus Nitrosopumilus maritimus,” a Cultivated Representative of the Cosmopolitan Mesophilic Group I Crenarchaeota

Citation
Schouten et al. (2008). Applied and Environmental Microbiology 74 (8)
Names
Ca. Nitrosopumilus maritimus
Abstract
ABSTRACT In this study we analyzed the membrane lipid composition of “ Candidatus Nitrosopumilus maritimus,” the only cultivated representative of the cosmopolitan group I crenarchaeota and the only mesophilic isolate of the phylum Crenarchaeota . The core lipids of “ Ca . Nitrosopumilus maritimus” consisted of glycerol dialkyl glycerol tetraethers (GDGTs) with zero to four cyclopentyl moieties. Crenar

Identification and Ecophysiological Characterization of Epiphytic Protein-Hydrolyzing Saprospiraceae (“ Candidatus Epiflobacter” spp.) in Activated Sludge

Citation
Xia et al. (2008). Applied and Environmental Microbiology 74 (7)
Names
Ca. Epiflobacter
Abstract
ABSTRACT The identity and ecophysiology of a group of uncultured protein-hydrolyzing epiphytic rods attached to filamentous bacteria in activated sludge from nutrient removal plants were investigated by using the full-cycle rRNA approach combined with microautoradiography and histochemical staining. The epiphytic group consists of three closely related clusters, each containing 11 to 16 clones. The closest related cultured isolate is the type strain Haliscomeno

Phylogenetic Characterization and Prevalence of “ Spirobacillus cienkowskii ,” a Red-Pigmented, Spiral-Shaped Bacterial Pathogen of Freshwater Daphnia Species

Citation
Rodrigues et al. (2008). Applied and Environmental Microbiology 74 (5)
Names
“Spirobacillus cienkowskii”
Abstract
ABSTRACT Microscopic examination of the hemolymph from diseased daphniids in 17 lakes in southwestern Michigan and five rock pools in southern Finland revealed the presence of tightly coiled bacteria that bore striking similarities to the drawings of a morphologically unique pathogen, “ Spirobacillus cienkowskii ,” first described by Elya Metchnikoff more than 100 years ago. The uncultivated microbe was identified as a deeply branching member of the

“ Candidatus Accumulibacter” Population Structure in Enhanced Biological Phosphorus Removal Sludges as Revealed by Polyphosphate Kinase Genes

Citation
He et al. (2007). Applied and Environmental Microbiology 73 (18)
Names
“Accumulibacter”
Abstract
ABSTRACT We investigated the fine-scale population structure of the “ Candidatus Accumulibacter” lineage in enhanced biological phosphorus removal (EBPR) systems using the polyphosphate kinase 1 gene ( ppk1 ) as a genetic marker. We retrieved fragments of “ Candidatus Accumulibacter” 16S rRNA and ppk1 genes from one laboratory-scale and several full-scale EBPR systems. Phylogeni

“ Candidatus Bacilloplasma,” a Novel Lineage of Mollicutes Associated with the Hindgut Wall of the Terrestrial Isopod Porcellio scaber (Crustacea: Isopoda)

Citation
Kostanjsek et al. (2007). Applied and Environmental Microbiology 73 (17)
Names
Ca. Bacilloplasma
Abstract
ABSTRACT Pointed, rod-shaped bacteria colonizing the cuticular surface of the hindgut of the terrestrial isopod crustacean Porcellio scaber (Crustacea: Isopoda) were investigated by comparative 16S rRNA gene sequence analysis and electron microscopy. The results of phylogenetic analysis, and the absence of a cell wall, affiliated these bacteria with the class Mollicutes , within which they represent a novel and deeply branche

Diversity of Nitrite Reductase Genes in “ Candidatus Accumulibacter phosphatis”-Dominated Cultures Enriched by Flow-Cytometric Sorting

Citation
Miyauchi et al. (2007). Applied and Environmental Microbiology 73 (16)
Names
“Accumulibacter phosphatis”
Abstract
ABSTRACT “ Candidatus Accumulibacter phosphatis” is considered a polyphosphate-accumulating organism (PAO) though it has not been isolated yet. To reveal the denitrification ability of this organism, we first concentrated this organism by flow cytometric sorting following fluorescence in situ hybridization (FISH) using specific probes for this organism. The purity of the target cells was about 97% of total cell count in the sorted sample. The PCR am

Evolutionary Relationships of “ Candidatus Riesia spp.,” Endosymbiotic Enterobacteriaceae Living within Hematophagous Primate Lice

Citation
Allen et al. (2007). Applied and Environmental Microbiology 73 (5)
Names
Ca. Riesia
Abstract
ABSTRACT The primary endosymbiotic bacteria from three species of parasitic primate lice were characterized molecularly. We have confirmed the characterization of the primary endosymbiont (P-endosymbiont) of the human head/body louse Pediculus humanus and provide new characterizations of the P-endosymbionts from Pediculus schaeffi from chimpanzees and Pthirus pubis , the pubic louse of humans. The endo

α-Proteobacterial Symbionts of Marine Bryozoans in the Genus Watersipora

Citation
Anderson, Haygood (2007). Applied and Environmental Microbiology 73 (1)
Names
“Endowatersipora glebosa” “Endowatersipora rubus”
Abstract
ABSTRACT Bacterial symbionts that resembled mollicutes were discovered in the marine bryozoan Watersipora arcuata in the 1980s. In this study, we used PCR and sequencing of 16S rRNA genes, specific fluorescence in situ hybridization, and phylogenetic analysis to determine that the bacterial symbionts of “ W. subtorquata ” and “ W. arcuata ” from several locations along the California coast are actually

Isolation, Pure Culture, and Characterization of “ Candidatus Arsenophonus arthropodicus,” an Intracellular Secondary Endosymbiont from the Hippoboscid Louse Fly Pseudolynchia canariensis

Citation
Dale et al. (2006). Applied and Environmental Microbiology 72 (4)
Names
Ca. Arsenophonus arthropodicus
Abstract
ABSTRACT Members of the genus Arsenophonus comprise a large group of bacterial endosymbionts that are widely distributed in arthropods of medical, veterinary, and agricultural importance. At present, little is known about the role of these bacteria in arthropods, because few representatives have been isolated and cultured in the laboratory. In the current study, we describe the isolation and pure culture of an Arsenophonus en