Search results (14)


Ornithodoros savignyi, the Tick Vector of “Candidatus Borrelia kalaharica” in Nigeria

Citation
Cutler et al. (2018). Journal of Clinical Microbiology 56 (9)
Names
Ca. Borrelia kalaharica
Subjects
Microbiology (medical)
Abstract
Endemic tick-borne relapsing fever (TBRF) has not been documented in Nigeria, yet clinically compatible cases have been described, and soft tick species are endemic in surrounding countries. Consequently, our aim was to investigate if TBRF-associated Borrelia is present in Nigeria.

Multilocus Sequence Analysis of Clinical “Candidatus Neoehrlichia mikurensis” Strains from Europe

Citation
Grankvist et al. (2015). Journal of Clinical Microbiology 53 (10)
Names
Ca. Neoehrlichia mikurensis Ca. Neoehrlichia
Subjects
Microbiology (medical)
Abstract
“CandidatusNeoehrlichia mikurensis” is the tick-borne agent of neoehrlichiosis, an infectious disease that primarily affects immunocompromised patients. So far, the genetic variability of “Ca. Neoehrlichia” has been studied only by comparing 16S rRNA genes andgroELoperon sequences. We describe the development and use of a multilocus sequence analysis (MLSA) protocol to characterize the genetic diversity of clinical “Ca. Neoehrlichia” strains in Europe and their relatedness to other species within theAnaplasmataceaefamily. Six genes were selected:ftsZ,clpB,gatB,lipA,groEL, and 16S rRNA. Each MLSA locus was amplified by real-time PCR, and the PCR products were sequenced. Phylogenetic trees of MLSA locus relatedness were constructed from aligned sequences. Blood samples from 12 patients with confirmed “Ca. Neoehrlichia” infection from Sweden (n= 9), the Czech Republic (n= 2), and Germany (n= 1) were analyzed with the MLSA protocol. Three of the Swedish strains exhibited identicallipAsequences, while thelipAsequences of the strains from the other nine patients were identical to each other. One of the Czech strains had one differing nucleotide in theclpBsequence from the sequences of the other 11 strains. All 12 strains had identical sequences for the genes 16S rRNA,ftsZ,gatB, andgroEL. According to the MLSA, among theAnaplasmataceae, “Ca. Neoehrlichia” is most closely related toEhrlichia ruminantium, less so toAnaplasma phagocytophilum, and least toWolbachiaendosymbionts. To conclude, three sequence types of infectious “Ca. Neoehrlichia” were identified: one in the west of Sweden, one in the Czech Republic, and one spread throughout Europe.

Molecular Typing of “Candidatus Bartonella ancashi,” a New Human Pathogen Causing Verruga Peruana

Citation
Mullins et al. (2013). Journal of Clinical Microbiology 51 (11)
Names
Ca. Bartonella ancashi
Subjects
Microbiology (medical)
Abstract
ABSTRACT A recently described clinical isolate, “ Candidatus Bartonella ancashi,” was obtained from a blood sample of a patient presenting with verruga peruana in the Ancash region of Peru. This sample and a second isolate obtained 60 days later from the same patient were molecularly typed using multilocus sequence typing (MLST) and multispacer sequence typing (MST). The isolates were 100% indistinguishable from each other but phylogenetically distant from Bartonella bacilliformis and considerably divergent from other known Bartonella species, confirming their novelty.

A Novel High-Resolution Melt PCR Assay Discriminates Anaplasma phagocytophilum and “Candidatus Neoehrlichia mikurensis”

Citation
Krucken et al. (2013). Journal of Clinical Microbiology 51 (6)
Names
Ca. Neoehrlichia mikurensis
Subjects
Microbiology (medical)
Abstract
ABSTRACT “ Candidatus Neoehrlichia mikurensis” ( Anaplasmataceae ) is an emerging pathogen transmitted by Ixodes ticks. Conventional PCR and the newly developed high-resolution melt PCR were used to detect and discriminate “ Candidatus Neoehrlichia mikurensis” and Anaplasma phagocytophilum . Both bacterial species were frequently found in Ixodes ricinus and Ixodes hexagonus but virtually absent from Dermacentor reticulatus . In rodents, “ Candidatus N. mikurensis” was significantly more prevalent than A. phagocytophilum , whereas in cats, only A. phagocytophilum was found.

Detection of “ Candidatus Neoehrlichia mikurensis” in Two Patients with Severe Febrile Illnesses: Evidence for a European Sequence Variant

Citation
von Loewenich et al. (2010). Journal of Clinical Microbiology 48 (7)
Names
Ca. Neoehrlichia mikurensis
Subjects
Microbiology (medical)
Abstract
ABSTRACT Recently, a new genus of Anaplasmataceae termed “ Candidatus Neoehrlichia” was discovered in ticks and rodents. Here, we report on two patients who suffered from febrile bacteremia due to “ Candidatus Neoehrlichia mikurensis” associated with thrombotic or hemorrhagic events. 16S rRNA and groEL gene sequencing provided evidence of three groups of sequence variants.