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Molecular Characterization of Mitogenome of Cacopsylla picta and Cacopsylla melanoneura, Two Vector Species of ‘Candidatus Phytoplasma mali’

Citation
Šafářová et al. (2023). Agronomy 13 (9)
Names
Ca. Phytoplasma mali
Abstract
The mitochondrial genomes of two vector psyllids of the ‘Candidatus Phytoplasma mali’, Cacopsylla picta and C. melanoneura, were sequenced using high-throughput sequencing on the Illumina platform. The main objective of the study was to describe their mitogenome and characterize their genetic variability and the potential changes in the context of the observed global warming. The four complete sequences for C. picta, 14,801 bp and 14,802 bp in length, two complete and one partial sequence for C.

Evaluation of the Effect of ‘Candidatus Liberibacter Solanacearum’ Haplotypes in Tobacco Infection

Citation
Levy et al. (2023). Agronomy 13 (2)
Names
“Liberibacter solanacearum” Liberibacter
Abstract
‘Candidatus Liberibacter solanacearum’ (Lso) is a phloem-limited bacterial plant pathogen infecting solanaceous plants in the Americas and New Zealand and is associated with diseases of apiaceous crops in Europe, Northern Africa, and the Middle East. This pathogen is also related to other Liberibacter species that infect other crops. In the USA, two haplotypes of Lso, LsoA and LsoB, are predominant and responsible for diseases in potato and tomato. Tobacco, Nicotiana tabacum, a model species to

Detection of ‘Candidatus Phythoplasma prunorum’ in Apricot Trees and its Associated Psyllid Samples

Citation
Koncz et al. (2023). Agronomy 13 (1)
Names
Ca. Phytoplasma prunorum Ca. Phythoplasma prunorum
Abstract
‘Candidatus Phytoplasma prunorum’ is causing ever increasing economic losses through the decline of apricot trees in European countries, e.g., Hungary. In this study, the pathogen was identified from plant tissues and insects by nested-PCR. The insect species were identified via morphology and molecular methods. The incidence of the pathogen was 29.6% in randomly selected apricot trees. Most of the infected trees with symptoms died within a year. These results show that phytoplasma is significan

Validation of Propidium Monoazide-qPCR for Assessing Treatment Effectiveness against ‘Candidatus Liberibacter asiaticus’ in Citrus

Citation
Yang, Ancona (2022). Agronomy 12 (11)
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing (HLB) is an important citrus disease associated with the phloem-limited, uncultured bacterium ‘Candidatus Liberibacter asiaticus’(CLas). Effective treatments against CLas have to be validated in the field, however, methods for the field assessment of treatment effectiveness are time-consuming, in part because DNA-based assays, including quantitative PCR (qPCR), cannot differentiate between live and dead bacterial DNA. The aim of this study was to develop a method for rapid the eval

Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable Infection

Citation
Cavichioli et al. (2022). Agronomy 12 (10)
Names
Ca. Liberibacter asiaticus
Abstract
Background: Huanglongbing (HLB) is currently one of the most devasting diseases in citrus plants worldwide. Resistance against its causal agent, Candidatus Liberibacter asiaticus (CLas), in commercial Citrus species remains a challenge, even though they show differences in CLas multiplication. Methods: A total of 14 citrandarins and their parents (Sunki mandarin and Poncirus trifoliata cv. Rubidoux) were top-grafted onto the canopy of potted ‘Valencia’ sweet orange plants with high CLas titers.

Association between Temperature and Reproductive Fitness of Diaphorina citri Infected with Candidatus Liberibacter Asiaticus

Citation
Hussain et al. (2022). Agronomy 12 (4)
Names
Ca. Liberibacter asiaticus Liberibacter
Abstract
Diaphorina citri is a serious insect pest of citrus and an insect vector of Candidatus Liberibacter asiaticus (CLas) that causes Huanglongbing disease (citrus greening). In this study, we investigated the effect of the CLas pathogen on the life history parameters of D. citri at different temperature regimes. Our results demonstrated that the survival rate of first to fifth instar CLas-positive and CLas-negative D. citri fluctuate with the change in temperature over the range of 16–35 °C. Meanwhi

Investigation of Citrus HLB Symptom Variations Associated with “Candidatus Liberibacter asiaticus” Strains Harboring Different Phages in Southern China

Citation
Bao et al. (2021). Agronomy 11 (11)
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing (HLB) is a devastating disease affecting citrus production worldwide. In China, the disease is associated with an unculturable alpha-proteobacterium, “Candidatus Liberibacter asiaticus” (CLas). Phages/prophages of CLas have recently been identified through intensive genomic research. The phage information has facilitated research on CLas biology such as population diversity and virulence gene identification. However, little is known about the roles of CLas phages in HLB symptom dev

Rootstock Influences on Health and Growth Following Candidatus Liberibacter asiaticus Infection in Young Sweet Orange Trees

Citation
Bowman, Albrecht (2020). Agronomy 10 (12)
Names
Ca. Liberibacter asiaticus
Abstract
Health, growth, fruit production, and fruit quality of citrus crops are severely affected by tree infection with Candidatus Liberibacter asiaticus (CLas) and subsequent development of the disease huanglongbing (HLB). The use of HLB-tolerant rootstocks is one strategy that is used to ameliorate the effects of HLB in commercial production. Although there is a clear long-term rootstock effect to improve tree performance, such field trials take many years for establishment and evaluation, and this l

Rootstock Influence on Growth and Mineral Content of Citrus limon and Citrus sinensis cv. Valencia Inoculated with Candidatus Liberibacter Asiaticus

Citation
Sáenz-Pérez et al. (2020). Agronomy 10 (10)
Names
Liberibacter Ca. Liberibacter asiaticus
Abstract
Huanglongbing (HLB) reduces the growth and development of citrus and induces changes in secondary metabolites such as flavonoids, limonoids, and polyamines. Likewise, infected plants have a deficient absorption of nutrients such as zinc, potassium, manganese, and copper. Therefore, the objective of this study was to evaluate the influence of different rootstocks on morphology and mineral changes of Citrus limon and Citrus sinensis cv. Valencia plants inoculated with Candidatus Liberibacter asiat