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Cloning of the spoT Gene of “ Candidatus Phlomobacter fragariae” and Development of a PCR-Restriction Fragment Length Polymorphism Assay for Detection of the Bacterium in Insects

Citation
Foissac et al. (2000). Applied and Environmental Microbiology 66 (8)
Names
Ca. Phlomobacter fragariae
Abstract
ABSTRACT Marginal chlorosis is a new disease of strawberry in which the uncultured phloem-restricted proteobacterium “ Candidatus Phlomobacter fragariae” is involved. In order to identify the insect(s) vector(s) of this bacterium, homopteran insects have been captured. Because a PCR test based on the 16S rRNA gene (rDNA) applied to these insects was unable to discriminate between “P. fragariae” and other insect-associated proteobacteria, isolation o