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Analysis of 525 Samples To Determine the Usefulness of PCR Amplification and Sequencing of the 16S rRNA Gene for Diagnosis of Bone and Joint Infections

Citation
Fenollar et al. (2006). Journal of Clinical Microbiology 44 (3)
Names
“Anaerococcus massiliensis” “Anaerococcus phoceensis” “Anaerococcus timonensis” “Clostridium massiliense” “Clostridium timonense” “Peptoniphilus massiliensis”
Abstract
ABSTRACT The 16S rRNA gene PCR in the diagnosis of bone and joint infections has not been systematically tested. Five hundred twenty-five bone and joint samples collected from 525 patients were cultured and submitted to 16S rRNA gene PCR detection of bacteria in parallel. The amplicons with mixed sequences were also cloned. When discordant results were observed, culture and PCR were performed once again. Bacteria were detected in 139 of 525 samples. Culture and 16S rRNA ge

Novel Genetic Variants of Anaplasma phagocytophilum , Anaplasma bovis , Anaplasma centrale , and a Novel Ehrlichia sp. in Wild Deer and Ticks on Two Major Islands in Japan

Citation
Kawahara et al. (2006). Applied and Environmental Microbiology 72 (2)
Names
“Ehrlichia shimanensis”
Abstract
ABSTRACT Wild deer are one of the important natural reservoir hosts of several species of Ehrlichia and Anaplasma that cause human ehrlichiosis or anaplasmosis in the United States and Europe. The primary aim of the present study was to determine whether and what species of Ehrlichia and Anaplasma naturally infect deer in Japan. Blood samples obtained from wild deer on two major

Tepidimicrobium ferriphilum gen. nov., sp. nov., a novel moderately thermophilic, Fe(III)-reducing bacterium of the order Clostridiales

Citation
Slobodkin et al. (2006). International Journal of Systematic and Evolutionary Microbiology 56 (2)
Names
Tepidimicrobium
Abstract
A moderately thermophilic, anaerobic bacterium (strain SB91T) was isolated from a freshwater hot spring at Barguzin Valley, Buryatiya, Russia. Cells of strain SB91T were straight to slightly curved rods, 0·5–0·6 μm in diameter and 3·0–7·0 μm in length. Formation of endospores was not observed. The temperature range for growth was 26–62 °C, with an optimum at 50 °C. The pH range for growth was 5·5–9·5, with an optimum at pH 7·5–8·0. The substrates utilized by strain SB91T in the presence of 9,10-