Publications (2826)

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Identification of salicylic acid binding proteins in the transcriptome of Citrus latifolia infected with Candidatus Liberibacter asiaticus

Citation
Santillán-Mendoza et al. (2023). Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology 40 (4)
Names
Ca. Liberibacter asiaticus
Subjects
General Medicine
Abstract
Persian lime (PL) is one of the most economically important citrus crops in the state of Veracruz, Mexico. However, it is affected by the presence of Huanglongbing (HLB) disease, caused by Candidatus Liberibacter asiaticus (CLas), an obligate biotrophic pathogen. Overall, PL shows a certain level of tolerance to HLB. Therefore, it is important to study the defense response mediated by salicylic acid (SA) against biotrophic pathogens in PL. Some genes with the capacity to participate in the SA response pathway, known as NtSABP, have been identified in Nicotiana tabacum, but the presence and activity of these genes in PL in response to HLB are unknown. The objective of this study was to identify homologues of SABP-like proteins in the PL transcriptome and to determine their differential expression level during CLas infection. SABP protein sequences from five different species, including N. tabacum, were used as model sequences in a tBLASTn search. A 3D model of the SABP protein was constructed and compared between N. tabacum and C. latifolia. We identified the direct homologous to each NtSABP gene in the PL using tBLASTn analysis, phylogenetic reconstruction, and tridimensional structure. Interestingly, the ClSABP1, ClSABP2, and ClSABP3 genes showed repression in CLas infected plants. There is at least one homologous to each NtSABP gene in PL. During CLas infection, these genes are somewhat suppressed.

The inside scoop: Comparative genomics of two intranuclear bacteria, “Candidatus Berkiella cookevillensis” and “Candidatus Berkiella aquae”

Citation
Kidane et al. (2022). PLOS ONE 17 (12)
Names
Ca. Berkiella cookevillensis Ca. Berkiella aquae
Subjects
Multidisciplinary
Abstract
“Candidatus Berkiella cookevillensis” (strain CC99) and “Candidatus Berkiella aquae” (strain HT99), belonging to the Coxiellaceae family, are gram-negative bacteria isolated from amoebae in biofilms present in human-constructed water systems. Both bacteria are obligately intracellular, requiring host cells for growth and replication. The intracellular bacteria-containing vacuoles of both bacteria closely associate with or enter the nuclei of their host cells. In this study, we analyzed the genome sequences of CC99 and HT99 to better understand their biology and intracellular lifestyles. The CC99 genome has a size of 2.9Mb (37.9% GC) and contains 2,651 protein-encoding genes (PEGs) while the HT99 genome has a size of 3.6Mb (39.4% GC) and contains 3,238 PEGs. Both bacteria encode high proportions of hypothetical proteins (CC99: 46.5%; HT99: 51.3%). The central metabolic pathways of both bacteria appear largely intact. Genes for enzymes involved in the glycolytic pathway, the non-oxidative branch of the phosphate pathway, the tricarboxylic acid pathway, and the respiratory chain were present. Both bacteria, however, are missing genes for the synthesis of several amino acids, suggesting reliance on their host for amino acids and intermediates. Genes for type I and type IV (dot/icm) secretion systems as well as type IV pili were identified in both bacteria. Moreover, both bacteria contain genes encoding large numbers of putative effector proteins, including several with eukaryotic-like domains such as, ankyrin repeats, tetratricopeptide repeats, and leucine-rich repeats, characteristic of other intracellular bacteria.