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An inhibitor-monitorable single-tube duplex quantitative real-time PCR assay for the detection of Candidatus Liberibacter asiaticus

Citation
Huang et al. (2023). Plant Disease
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing (HLB) is a citrus infectious disease caused by Candidatus Liberibacters spp. Recently, it has begun to spread rapidly worldwide, causing significant losses to the citrus industry. Early diagnosis of HLB relies on quantitative real-time PCR assays. However, the PCR inhibitors found in the nucleic acid extracted from plant materials pose challenges for PCR assays because they may result in false-negative results. Internal standard (IS) can be introduced to establish a single-tube du

A comprehensive overview of the Chloroflexota community in wastewater treatment plants worldwide

Citation
Petriglieri et al. (2023). mSystems 8 (6)
Names
“Epilinea brevis” “Epilinea” “Leptofilum” “Epilineaceae” “Epilineales” “Avedoeria danica” “Avedoeria” “Brachythrix odensensis” “Brachythrix” “Defluviilinea gracilis” “Defluviilinea” “Defluviilinea proxima” “Villigracilis vicinus” “Villigracilis adiacens” “Villigracilis propinquus” “Villigracilis” “Villigracilis affinis” “Villigracilis proximus” “Villigracilis saccharophilus” “Villigracilaceae” “Hadersleviella danica” “Hadersleviella” “Trichofilum aggregatum” “Trichofilum” “Promineofilum glycogenicum” “Leptofilum gracile” “Leptofilum proximum” “Leptovillus gracilis” “Leptovillus affinis” “Leptovillus” “Flexicrinis affinis” “Flexicrinis proximus” “Flexicrinis” “Flexifilum breve” “Flexifilum affine” “Flexifilum” “Flexifilaceae” “Amarolinea dominans” “Fredericiella danica” “Fredericiella” “Caldilinea saccharophila” “Ribeiella danica” “Ribeiella” “Kouleothrix ribensis” “Amarobacter glycogenicus” “Amarobacter” “Amarobacillus elongatus” “Amarobacillus”
Abstract
ABSTRACT Filamentous Chloroflexota are abundant in activated sludge wastewater treatment plants (WWTPs) worldwide and are occasionally associated with poor solid-liquid separation or foaming, but most of the abundant lineages remain undescribed. Here, we present a comprehensive overview of Chloroflexota abundant in WWTPs worldwide, using high-quality metagenome-assembled genomes (MAGs) and 16S rRNA amplicon data from 740 Danish and global WWTPs. Many novel taxa were descri

Comparative genomics of symbiotic Photobacterium using highly contiguous genome assemblies from long read sequences

Citation
Gould, Henderson (2023). Microbial Genomics 9 (12)
Names
“Photobacterium acropomis”
Abstract
This study presents the assembly and comparative genomic analysis of luminous Photobacterium strains isolated from the light organs of 12 fish species using Oxford Nanopore Technologies (ONT) sequencing. The majority of assemblies achieved chromosome-level continuity, consisting of one large (>3 Mbp) and one small (~1.5 Mbp) contig, with near complete BUSCO scores along with varying plasmid sequences. Leve

Accumulation and Transmission of ‘Candidatus Liberibacter solanacearum’ Haplotypes by the Nymphs of Two Psyllid Vectors

Citation
Oh et al. (2023). Insects 14 (12)
Names
“Liberibacter solanacearum”
Abstract
‘Candidatus Liberibacter solanacearum’ (Lso) is a plant pathogenic bacterium transmitted by psyllids that causes significant agricultural damage. Several Lso haplotypes have been reported. Among them, LsoA and LsoB are transmitted by the potato psyllid Bactericera cockerelli and infect solanaceous crops, and LsoD is transmitted by the carrot psyllid B. trigonica and infects apiaceous crops. Several studies evaluated the transmission of these haplotypes by adult psyllids. However, fewer data are

Exploration of biotic inducers through seed infiltration technique for the management of phyllody in sesame caused by Candidatus Phytoplasma

Citation
M KOWSALYA et al. (2023). Journal of Oilseeds Research 40 (Specialissue)
Names
Ca. Phytoplasma
Abstract
This study aimed to evaluate the efficacy of biotic inducers for the management of phyllody disease in sesame, as well as in analyzing the defense enzyme activity. The field trial experiments were conducted with the biotic inducers [salicylic acid (SA), methyl jasmonate (MeJA), and beta amino butyric acid (BABA)] in individually (50 ppm, 100 ppm and 150 ppm) and in combination. Concerning biotic inducers, SA 50 / 100 ppm pre-seed treatment and exogenous application at 30th, 45th and 60th DAS res

Draft Genome of Nocardia canadensis sp. nov. Isolated from Petroleum-Hydrocarbon-Contaminated Soil

Citation
Alotaibi et al. (2023). Microorganisms 11 (12)
Names
Nocardia canadensis
Abstract
The bacterial strain WB46 was isolated from the rhizosphere of willow plants (Salix purpurea L.) growing in soil contaminated with petroleum hydrocarbons. The strain was subjected to whole-genome shotgun sequencing using Illumina HiSeq. Its draft genome is 7.15 Mb, with a 69.55% GC content, containing 6387 protein-coding genes and 51 tRNA and 15 rRNA sequences. The quality and reliability of the genome were assessed using CheckM, attaining an estimated genome completeness of 98.75% and an estima