The sequence of the genome of “
Tremblaya princeps” strain PCVAL, the primary endosymbiont of the citrus mealybug
, has been determined. “
. Tremblaya princeps” presents an unusual nested endosymbiosis and harbors a gammaproteobacterial symbiont within its cytoplasm in all analyzed mealybugs. The genome sequence reveals that “
. Tremblaya princeps” cannot be considered an independent organism but that the consortium with its gammaproteobacterial symbiotic associate represents a new composite living being.