Pimenov, Nikolay V.


Publications
3

Metabolic Diversity and Evolutionary History of the Archaeal Phylum “ Candidatus Micrarchaeota” Uncovered from a Freshwater Lake Metagenome

Citation
Kadnikov et al. (2020). Applied and Environmental Microbiology 86 (23)
Names
“Fermentimicrarchaeaceae” “Fermentimicrarchaeales” Ca. Micrarchaeota Ca. Diapherotrites
Abstract
The recently described superphylum DPANN includes several phyla of uncultivated archaea with small cell sizes, reduced genomes, and limited metabolic capabilities. One of these phyla, “ Ca . Micrarchaeota,” comprises an enigmatic group of archaea found in acid mine drainage environments, the archaeal Richmond Mine acidophilic nanoorganisms (ARMAN) group. Analysis of their reduced genomes revealed the absence of key metabolic pathways consistent with their par

A Simple and Rapid System for Proteomic Analysis of the Archaeon Candidatus Vulcanisaeta moutnovskia

Citation
Chernyh et al. (2020).
Names
Ca. Vulcanisaeta moutnovskia
Abstract
Abstract This protocol describes a rapid protein extraction method for the archaeon Candidatus Vulcanisaeta moutnovskia, which can be also implemented for other archaea. The utilization of two different methods for protein extraction constitute the main step of the protocol. Method I involves the extraction with a multi-chaotropic lysis buffer containing a non-denaturing zwitterionic detergent, most efficient for extracting cytosolic proteins. Method II involves a denaturing anionic dete