Heck, Michelle


Publications
16

Grove-level analysis of titer and prevalence of “CandidatusLiberibacter asiaticus” andWolbachiainDiaphorina citri,vector of citrus Huanglongbing

Citation
Mann et al. (2023).
Names
Abstract
AbstractHuanglongbing (HLB, or citrus greening disease) affects all citrus varieties world-wide. In the USA, Asia, and South America the causal agent is “CandidatusLiberibacter asiaticus” (CLas), a phloem-limited, uncultured, alphaproteobacterium. The hemipteran insect vector,Diaphorina citri(Asian citrus psyllid) acquires and transmitsCLas in a circulative, propagative manner. In addition toCLas,D. citrihosts multiple symbiotic bacteria includingWolbachia(wDi). InD. citri, wDi has been sequence

A Perspective on Current Therapeutic Molecule Screening Methods Against ‘Candidatus Liberibacter asiaticus’, the Presumed Causative Agent of Citrus Huanglongbing

Citation
Kennedy et al. (2023). Phytopathology® 113 (7)
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing (HLB), referred to as citrus greening disease, is a bacterial disease impacting citrus production worldwide and is fatal to young trees and mature trees of certain varieties. In some areas, the disease is devastating the citrus industry. A successful solution to HLB will be measured in economics: citrus growers need treatments that improve tree health, fruit production, and most importantly, economic yield. The profitability of citrus groves is the ultimate metric that truly matte

Plant-derived, nodule-specific cysteine rich peptides inhibit growth and psyllid acquisition of ‘CandidatusLiberibacter asiaticus’, the citrus Huanglongbing bacterium

Citation
Higgins et al. (2023).
Names
Abstract
AbstractThe Asian citrus psyllid,Diaphorina citri, is a vector of ‘CandidatusLiberibacter asiaticus’ (CLas), a gram-negative, obligate biotroph whose infection inCitrusspecies is associated with citrus greening disease, or Huanglongbing (HLB). Strategies to blockCLas transmission byD. citriremain the best way to prevent the spread of the disease into new citrus growing regions. However, identifying control strategies to block HLB transmission poses significant challenges, such as the discovery a

Strain Tracking of ‘Candidatus Liberibacter asiaticus’, the Citrus Greening Pathogen, by High-Resolution Microbiome Analysis of Asian Citrus Psyllids

Citation
Higgins et al. (2022). Phytopathology® 112 (11)
Names
Ca. Liberibacter asiaticus
Abstract
The Asian citrus psyllid, Diaphorina citri, is an invasive insect and a vector of ‘ Candidatus Liberibacter asiaticus’ ( CLas), a bacterium whose growth in Citrus species results in huanglongbing (HLB), also known as citrus greening disease. Methods to enrich and sequence CLas from D. citri often rely on biased genome amplification and nevertheless contain significant quantities of host DNA. To overcome these hurdles, we developed a simple pretreatment DNase and filtration (PDF) protocol to rem

Direct DNA sequencing of ‘Candidatus Liberibacter asiaticus’ from Diaphorina citri, the Asian citrus psyllid, and its implications for citrus greening disease management

Citation
Higgins et al. (2022).
Names
Ca. Liberibacter asiaticus
Abstract
AbstractThe Asian citrus psyllid, Diaphorina citri is an invasive insect 1 and a vector of ’Candidatus Liberibacter asiaticus’ (CLas), a bacterium whose growth in Citrus species results in citrus greening disease 2,3. Methods to enrich and sequence CLas from D. citri often rely on biased genome amplification 4 and nevertheless contain significant quantities of host DNA 5,6. To overcome these hurdles, we developed a simple pre-treatment DNase and filtration (hereafter PDF) protocol to directly se

Host Plant Adaptation Drives Changes in Diaphorina citri Proteome Regulation, Proteoform Expression, and Transmission of ‘Candidatus Liberibacter asiaticus’, the Citrus Greening Pathogen

Citation
Ramsey et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
The Asian citrus psyllid (Diaphorina citri) is a pest of citrus and the primary insect vector of the bacterial pathogen, ‘Candidatus Liberibacter asiaticus’ (CLas), which is associated with citrus greening disease. The citrus relative Murraya paniculata (orange jasmine) is a host plant of D. citri but is more resistant to CLas compared with all tested Citrus genotypes. The effect of host switching of D. citri between Citrus medica (citron) and M. paniculata plants on the acquisition and transmi

An Excised Leaf Assay to Measure Acquisition of ‘Candidatus Liberibacter asiaticus’ by Psyllids Associated with Citrus Huanglongbing Disease

Citation
Igwe et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing, or citrus greening disease, is the most serious disease of citrus worldwide and is associated with plant infection by ‘Candidatus Liberibacter asiaticus’ (CLas) and other Liberibacter species. CLas is transmitted by Diaphorina citri, the Asian citrus psyllid, in a circulative propagative manner. Circulative propagative transmission is a complex process comprising at least three steps: movement of the pathogen into vector tissues, translocation and replication of the pathogen with

Multi-omics Comparison Reveals Landscape of Citrus limon and Citrus sinensis Response to ‘Candidatus Liberibacter asiaticus’

Citation
Chin et al. (2021). PhytoFrontiers™ 1 (2)
Names
Ca. Liberibacter asiaticus
Abstract
Comparison of the metabolic changes prior to symptom development upon infection with Candidatus Liberibacter asiaticus (CLas), the bacterium associated with citrus greening disease, between citrus hosts with different tolerances is lacking. The objective of this study was to compare the early response of Lisbon lemon (Citrus limon) and Washington navel orange (Citrus sinensis [L.] Osbeck), two citrus species commercially important to California, to CLas through graft inoculation. Here, we compa