Chen, Jianchi


Publications
22

Enhancing PCR Capacity To Detect ‘Candidatus Liberibacter asiaticus’ Utilizing Whole Genome Sequence Information

Citation
Bao et al. (2020). Plant Disease 104 (2)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’ (CLas) is an unculturable α-proteobacterium associated with citrus Huanglongbing (HLB; yellow shoot disease). PCR procedures that accurately confirm or exclude CLas infection in citrus tissue/Asian citrus psyllid (ACP) samples are critical for HLB management. When CLas was described in 1994, a 23-bp signature oligonucleotide sequence (OI1) in the 16S rRNA gene (rrs, three genomic copies) was identified based on Sanger sequencing. OI1 contains single nucleotid

Draft Genome Sequence of “ Candidatus Sulcia muelleri” Strain KPTW1 from Kolla paulula , a Vector of Xylella fastidiosa Causing Pierce’s Disease of Grapevine in Taiwan

Citation
Shih et al. (2019). Microbiology Resource Announcements 8 (2)
Names
Ca. Sulcia muelleri
Abstract
The genome of “ Candidatus Sulcia muelleri” strain KPTW1 from Kolla paulula , a vector of Xylella fastidiosa that causes Pierce’s disease (PD) of grapevine in Taiwan, was sequenced. The strain has a genome size of 253,942 bp, GC content of 22.7%, 237 predicted protein-coding genes, and 34 RNA genes.

A Type 3 Prophage of ‘Candidatus Liberibacter asiaticus’ Carrying a Restriction-Modification System

Citation
Zheng et al. (2018). Phytopathology® 108 (4)
Names
Ca. Liberibacter asiaticus
Abstract
Prophages, the lysogenic form of bacterial phages, are important genetic entities of ‘Candidatus Liberibacter asiaticus’ (CLas), a nonculturable α-proteobacterium associated with citrus Huanglongbing. Two CLas prophages have been described, SC1 (NC_019549.1, Type 1) and SC2 (NC_019550.1, Type 2), which involve the lytic cycle and the lysogenic cycle, respectively. To explore the prophage repertoire, 523 CLas DNA samples extracted from leaf petioles of CLas-infected citrus were collected from so

Draft Whole-Genome Sequence of “ Candidatus Liberibacter asiaticus” Strain TX2351 Isolated from Asian Citrus Psyllids in Texas, USA

Citation
Kunta et al. (2017). Genome Announcements 5 (15)
Names
Ca. Liberibacter asiaticus
Abstract
ABSTRACT We report here the draft genome sequence of “ Candidatus Liberibacter asiaticus” strain TX2351, collected from Asian citrus psyllids in south Texas, USA. The TX2351 genome has a size of 1,252,043 bp, a G+C content of 36.5%, 1,184 predicted open reading frames, and 52 RNA genes.

Molecular characterization of a mosaic locus in the genome of 'CandidatusLiberibacter asiaticus'

Citation
Wang et al. (2012). BMC Microbiology 12 (1)
Names
Ca. Liberibacter asiaticus
Abstract
Abstract Background Huanglongbing (HLB) is a highly destructive disease of citrus production worldwide. 'Candidatus Liberibacter asiaticus', an unculturable alpha proteobacterium, is a putative pathogen of HLB. Information about the biology and strain diversity of 'Ca. L. asiaticus' is currently limited, inhibiting the scope of HLB research and control. Results A genomic region (CLIBASIA_05640 to CLIBASIA_05650) of

Analysis of a Prophage Gene Frequency Revealed Population Variation of ‘Candidatus Liberibacter asiaticus’ from Two Citrus-Growing Provinces in China

Citation
Liu et al. (2011). Plant Disease 95 (4)
Names
Ca. Liberibacter asiaticus
Abstract
Prophages are important genetic elements of bacterial genomes and are involved in lateral gene transfer, pathogenicity, environmental adaptations, and interstrain genetic variability. In this study, the sequence of a prophage terminase gene of ‘Candidatus Liberibacter asiaticus’, a bacterium associated with citrus Huanglongbing (HLB), was selected as a molecular marker to assess the genetic variation in two ‘Ca. L. asiaticus’ populations from geographically distinct provinces (Guangdong and Yun