Lopes, Silvio A.


Publications
6

Protocol for Successful Transmission of ‘Candidatus Liberibacter asiaticus’ from Citrus to Citrus Using Diaphorina citri

Citation
Lopes et al. (2021). Phytopathology® 111 (12)
Names
Ca. Liberibacter asiaticus
Abstract
A protocol to successfully transmit the huanglongbing (HLB) pathogen, ‘Candidatus Liberibacter asiaticus’, between citrus plants by using the Asian citrus psyllid (ACP) and an alternative way to help growers control ACP are proposed. Best results were obtained when pathogen acquisition by adults reared on fully symptomatic ‘Ca. Liberibacter asiaticus’-positive plants, latency, and inoculation occurred at ambient air temperatures ranging from 24 to 28°C and when a single infective adult ACP was

Modeling ‘Candidatus Liberibacter asiaticus’ Movement Within Citrus Plants

Citation
Raiol Junior et al. (2021). Phytopathology® 111 (10)
Names
Ca. Liberibacter asiaticus
Abstract
The phloem-limited ‘Candidatus Liberibacter asiaticus’ (Las) causes huanglongbing, a destructive citrus disease. Graft-inoculated potted plants were used to assess Las speed of movement in phloem in the greenhouse, and the impacts of temperature on plant colonization in growth-chamber experiments. For assessment of Las speed, plants were inoculated at the main stem and assessed over time by quantitative PCR (qPCR) or symptoms at various distances from the inoculum. For colonization, the plants

Resistance to ‘Candidatus Liberibacter asiaticus,’ the Huanglongbing Associated Bacterium, in Sexually and/or Graft-Compatible Citrus Relatives

Citation
Alves et al. (2021). Frontiers in Plant Science 11
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing (HLB) is the most destructive, yet incurable disease of citrus. Finding sources of genetic resistance to HLB-associated ‘Candidatus Liberibacter asiaticus’ (Las) becomes strategic to warrant crop sustainability, but no resistant Citrus genotypes exist. Some Citrus relatives of the family Rutaceae, subfamily Aurantioideae, were described as full-resistant to Las, but they are phylogenetically far, thus incompatible with Citrus. Partial resistance was indicated for certain cross-comp

Evidence That ‘Candidatus Liberibacter asiaticus’ Moves Predominantly Toward New Tissue Growth in Citrus Plants

Citation
Raiol-Junior et al. (2021). Plant Disease 105 (1)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’ (Las) is an unculturable, phloem-limited, insect-transmitted bacterium associated with the Asiatic form of huanglongbing (HLB), the most destructive citrus disease. In Asia and the Americas, it is transmitted by the Asian citrus psyllid (Diaphorina citri Kuwavama). Despite considerable research, little is known about the processes involved in plant infection and colonization by Las. This study was conducted to determine whether the basal portion (below girdli

Seasonal Variation of ‘Candidatus Liberibacter asiaticus’ Titers in New Shoots of Citrus in Distinct Environments

Citation
Lopes et al. (2017). Plant Disease 101 (4)
Names
Ca. Liberibacter asiaticus
Abstract
The major citrus area of Brazil occupies near 450,000 ha between the Triângulo Mineiro (TM) region of Minas Gerais State and the south of São Paulo State (SPS). Significant climatic variation occurs between regions which could affect huanglongbing (HLB) progress, which is lower in TM. To investigate this possibility, young sweet orange shoots were sampled periodically over 2 years to determine ‘Candidatus Liberibacter asiaticus’ titers in naturally infected trees in orchards in Analândia, centr

Improvement in the Excised Citrus Leaf Assay to Investigate Inoculation of ‘Candidatus Liberibacter asiaticus’ by the Asian Citrus Psyllid Diaphorina citri

Citation
Raiol-Junior et al. (2017). Plant Disease 101 (3)
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing (HLB) is a difficult-to-control and highly destructive citrus disease that, in Brazil, is associated mainly with the bacterium ‘Candidatus Liberibacter asiaticus’ transmitted by the psyllid Diaphorina citri. The aim of this study was to improve our understanding of the ‘Ca. L. asiaticus’ infection process by exposing excised, fully expanded, immature citrus leaves in 50-ml Falcon tubes to one, four, or eight adults from a ‘Ca. L. asiaticus’-exposed colony for 1-, 3-, 7-, or 15-day