Yadav, Vandana


Molecular Detection of Candidatus Phytoplasma Trifolii Associated with Little Leaf of Brinjal from Kerala State of Southern India

Yadav et al. (2015). International Journal of Life Sciences 9 (6)
Ca. Phytoplasma
rinjal is an important vegetable crop grown worldwide. A field survey conducted during 2012 in Kerala state of South India, the little leaf disease affected brinjal plant was observed in one of the home gardens located at Nemmara (Palakkad district). The diseased and healthy samples were collected; genomic DNA was isolated and 16SrRNA gene amplified by performing nested PCR using universal primers pairs P1/P7 and R16F2n/R2 respectively. The association of Phytoplasma was detected in all the thre