Smith, Richard D.


Publications
4

Proteome Remodeling in Response to Sulfur Limitation in “ Candidatus Pelagibacter ubique”

Citation
Smith et al. (2016). mSystems 1 (4)
Names
Ca. Pelagibacter ubique
Abstract
“ Ca . Pelagibacter ubique” is a key driver of marine biogeochemistry cycles and a model for understanding how minimal genomes evolved in free-living anucleate organisms. This study explores the unusual sulfur acquisition strategy that has evolved in these cells, which lack assimilatory sulfate reduction and instead rely on reduced sulfur compounds found in oxic marine environments to meet their cellular quotas. Our findings demonstrate that the sulfur acquis

Proteomic and Transcriptomic Analyses of “ Candidatus Pelagibacter ubique” Describe the First P II -Independent Response to Nitrogen Limitation in a Free-Living Alphaproteobacterium

Citation
Smith et al. (2013). mBio 4 (6)
Names
Ca. Pelagibacter ubique
Abstract
ABSTRACT Nitrogen is one of the major nutrients limiting microbial productivity in the ocean, and as a result, most marine microorganisms have evolved systems for responding to nitrogen stress. The highly abundant alphaproteobacterium “ Candidatus Pelagibacter ubique,” a cultured member of the order Pelagibacterales (SAR11), lacks the canonical GlnB, GlnD, GlnK, and NtrB/NtrC genes for regulating nitrogen assimilation, raisin

Proteomic Analysis of Stationary Phase in the Marine Bacterium “ Candidatus Pelagibacter ubique”

Citation
Sowell et al. (2008). Applied and Environmental Microbiology 74 (13)
Names
Ca. Pelagibacter ubique
Abstract
ABSTRACT “ Candidatus Pelagibacter ubique,” an abundant marine alphaproteobacterium, subsists in nature at low ambient nutrient concentrations and may often be exposed to nutrient limitation, but its genome reveals no evidence of global regulatory mechanisms for adaptation to stationary phase. High-resolution capillary liquid chromatography coupled online to an LTQ mass spectrometer was used to build an accurate mass and time (AMT) tag library that