Smith, Grant R.


Publications
6

Genetic drift and genome reduction in the plant pathogenCandidatusLiberibacter solanacearum shapes a new enzyme in lysine biosynthesis

Citation
Gilkes et al. (2023).
Names
“Liberibacter solanacearum”
Abstract
AbstractThe effect of population bottlenecks and genome reduction on enzyme function is poorly understood. ‘CandidatusLiberibacter solanacearum’ is a bacterium with a reduced genome that is transmitted vertically to the egg of an infected psyllid—a population bottleneck that imposes genetic drift and is predicted to affect protein structure and function. Here, we define the effects of genome reduction and genetic drift on the function ofCa. L. solanacearum dihydrodipicolinate synthase (CLsoDHDPS

The First Purification of Functional Proteins from the Unculturable, Genome-Reduced, Bottlenecked α-Proteobacterium ‘Candidatus Liberibacter solanacearum’

Citation
Gilkes et al. (2019). Phytopathology® 109 (7)
Names
“Liberibacter solanacearum”
Abstract
‘Candidatus Liberibacter solanacearum’ is an unculturable α-proteobacterium that is the causal agent of zebra chip disease of potato—a major problem in potato-growing areas, because it affects growth and yield. Developing effective treatments for ‘Ca. L. solanacearum’ has been hampered by the difficulty in functionally characterizing the proteins of this organism, largely because they are not easily expressed and purified in standard expression systems. ‘Ca. L. solanacearum’ has a reduced genom

Draft Genome Sequence of a “ Candidatus Liberibacter europaeus” Strain Assembled from Broom Psyllids (Arytainilla spartiophila) from New Zealand

Citation
Frampton et al. (2018). Genome Announcements 6 (20)
Names
Ca. Liberibacter europaeus
Abstract
ABSTRACT Here, we report the draft genome sequence of “ Candidatus Liberibacter europaeus” ASNZ1, assembled from broom psyllids ( Arytainilla spartiophila ) from New Zealand. The assembly comprises 15 contigs, with a total length of 1.33 Mb and a G+C content of 33.5%.

Genomes of ‘Candidatus Liberibacter solanacearum’ Haplotype A from New Zealand and the United States Suggest Significant Genome Plasticity in the Species

Citation
Thompson et al. (2015). Phytopathology® 105 (7)
Names
“Liberibacter solanacearum”
Abstract
‘Candidatus Liberibacter solanacearum’ contains two solanaceous crop-infecting haplotypes, A and B. Two haplotype A draft genomes were assembled and compared with ZC1 (haplotype B), revealing inversion and relocation genomic rearrangements, numerous single-nucleotide polymorphisms, and differences in phage-related regions. Differences in prophage location and sequence were seen both within and between haplotype comparisons. OrthoMCL and BLAST analyses identified 46 putative coding sequences pre