Peng, Yongzhen


Publications (11)

Population Structure and Morphotype Analysis of “ Candidatus Accumulibacter” Using Fluorescence In Situ Hybridization-Staining-Flow Cytometry

Citation
Li et al. (2019). Applied and Environmental Microbiology 85 (9)
Names
Ca. Accumulibacter
Subjects
Applied Microbiology and Biotechnology Biotechnology Ecology Food Science
Abstract
As one group of the most important functional phosphorus removal organisms, “ Candidatus Accumulibacter,” affiliated with the Rhodocyclus group of the Betaproteobacteria , is a widely recognized and studied PAO in the field of biological wastewater treatment. The morphotypes and population structure of clade-level “ Candidatus Accumulibacter” were studied through novel FISH-staining-flow cytometry, which involved denitrifying phosphorus removal (DPR) achieving carbon and energy savings and simultaneous removal of N and P, thus inferring the different denitrifying phosphorus removal abilities of these clades. Additionally, based on this method, in situ quantification for specific polyphosphate-accumulating organisms (PAOs) enables a more efficient process and more accurate result. The establishment of FISH-staining-flow cytometry makes cell sorting of clade-level noncultivated organisms available.