Leutenegger, Christian M.


Detection of Mixed Infections with “Candidatus Mycoplasma Haemominutum” and Mycoplasma Haemofelis Using Real-Time TaqMan Polymerase Chain Reaction

Sykes et al. (2007). Journal of Veterinary Diagnostic Investigation 19 (3)
The objective of this study was to evaluate the use of real-time TaqMan PCR assays for detection of coinfections with “ Candidatus Mycoplasma haemominutum” (Mhm), and Mycoplasma haemofelis (Mhf), in vitro and over time in experimentally infected cats. First, the ability of each real-time PCR assay to detect and quantify mixed infections was determined in vitro by testing mixtures of plasmids containing Mhm and Mhf 16S rDNA with each assay. Subsequently, 4 specific pathogen-free (SPF) cats, 2 of