Huang, Weida


An inhibitor-monitorable single-tube duplex quantitative real-time PCR assay for the detection of Candidatus Liberibacter asiaticus

Huang et al. (2023). Plant Disease
Ca. Liberibacter asiaticus
Huanglongbing (HLB) is a citrus infectious disease caused by Candidatus Liberibacters spp. Recently, it has begun to spread rapidly worldwide, causing significant losses to the citrus industry. Early diagnosis of HLB relies on quantitative real-time PCR assays. However, the PCR inhibitors found in the nucleic acid extracted from plant materials pose challenges for PCR assays because they may result in false-negative results. Internal standard (IS) can be introduced to establish a single-tube du