Khomyakova, Maria A.


Publications
2

Phenotypic and genomic characterization of the first alkaliphilic aceticlastic methanogens and proposal of a novel genus Methanocrinis gen.nov. within the family Methanotrichaceae

Citation
Khomyakova et al. (2023). Frontiers in Microbiology 14
Names
Methanocrinis alkalitolerans Methanocrinis natronophilus Methanocrinis harundinaceus Ts Methanocrinis
Abstract
Highly purified cultures of alkaliphilic aceticlastic methanogens were collected for the first time using methanogenic enrichments with acetate from a soda lake and a terrestrial mud volcano. The cells of two strains were non-motile rods forming filaments. The mud volcano strain M04Ac was alkalitolerant, with the pH range for growth from 7.5 to 10.0 (optimum at 9.0), while the soda lake strain Mx was an obligate alkaliphile growing in the pH range 7.7–10.2 (optimum 9.3–9.5) in the presence of op

Phenotypic and genomic characterization of Bathyarchaeum tardum gen. nov., sp. nov., a cultivated representative of the archaeal class Bathyarchaeia

Citation
Khomyakova et al. (2023). Frontiers in Microbiology 14
Names
Bathyarchaeum tardum Ts Bathyarchaeum Bathyarchaeia Bathyarchaeales Bathyarchaeaceae Methanocrinis Methanocrinis harundinaceus Ts Methanocrinis alkalitolerans Methanocrinis natronophilus
Abstract
Bathyarchaeia are widespread in various anoxic ecosystems and are considered one of the most abundant microbial groups on the earth. There are only a few reports of laboratory cultivation of Bathyarchaeia, and none of the representatives of this class has been isolated in pure culture. Here, we report a sustainable cultivation of the Bathyarchaeia archaeon (strain M17CTs) enriched from anaerobic sediment of a coastal lake. The cells of strain M17CTs were small non-motile cocci, 0.4–0.7 μm in dia