Adame-García, Jacel

Publications (1)

Identification of salicylic acid binding proteins in the transcriptome of Citrus latifolia infected with Candidatus Liberibacter asiaticus

Santillán-Mendoza et al. (2023). Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology 40 (4)
Ca. Liberibacter asiaticus
General Medicine
Persian lime (PL) is one of the most economically important citrus crops in the state of Veracruz, Mexico. However, it is affected by the presence of Huanglongbing (HLB) disease, caused by Candidatus Liberibacter asiaticus (CLas), an obligate biotrophic pathogen. Overall, PL shows a certain level of tolerance to HLB. Therefore, it is important to study the defense response mediated by salicylic acid (SA) against biotrophic pathogens in PL. Some genes with the capacity to participate in the SA response pathway, known as NtSABP, have been identified in Nicotiana tabacum, but the presence and activity of these genes in PL in response to HLB are unknown. The objective of this study was to identify homologues of SABP-like proteins in the PL transcriptome and to determine their differential expression level during CLas infection. SABP protein sequences from five different species, including N. tabacum, were used as model sequences in a tBLASTn search. A 3D model of the SABP protein was constructed and compared between N. tabacum and C. latifolia. We identified the direct homologous to each NtSABP gene in the PL using tBLASTn analysis, phylogenetic reconstruction, and tridimensional structure. Interestingly, the ClSABP1, ClSABP2, and ClSABP3 genes showed repression in CLas infected plants. There is at least one homologous to each NtSABP gene in PL. During CLas infection, these genes are somewhat suppressed.